Planta Med 2011; 77(4): 322-327
DOI: 10.1055/s-0030-1250406
Biological and Pharmacological Activity
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Annomontine, an Alkaloid Isolated from Annona purpurea, Has Anxiolytic-Like Effects in the Elevated Plus-Maze

José del Carmen Rejón-Orantes1 , Alma Rosa González-Esquinca2 , Miguel Pérez de la Mora3 , Gabriel Roldan Roldan4 , Diego Cortes5
  • 1Laboratorio Experimental de Farmacobiología, Facultad de Medicina, Universidad Autónoma de Chiapas, Tuxtla Gutiérrez, Mexico
  • 2Laboratorio de Fisiología y Química Vegetal, Facultad de Ciencias Biológicas, Universidad de Ciencias y Artes de Chiapas, Tuxtla Gutiérrez, Mexico
  • 3Departamento de Biofísica, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico
  • 4Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico
  • 5Facultad de Farmacia, Departamento de Farmacología de la Universidad de Valencia, Valencia, Spain
Further Information

Publication History

received June 26, 2010 revised Sept. 4, 2010

accepted Sept. 10, 2010

Publication Date:
01 October 2010 (online)

Abstract

The effects of annomontine, a pyrimidine-β-carboline alkaloid isolated from the root of Annona purpurea, on anxiety was studied in mice using the elevated plus-maze. The behavioral effects of this alkaloid on the pentobarbital-induced hypnosis, the locomotor activity in an open field, and the motor coordination in the rotarod test were also evaluated. The intraperitoneal injection of annomontine (1–30 mg/kg) increased in a dose-dependent way the number of visits to and the time spent in the open arms of the elevated plus-maze in comparison to the control animals. Such effects were blocked by the prior application of flumazenil (3 mg/kg; i. p.), a specific antagonist for the binding of benzodiazepines on the GABAA receptor. Under the same experimental conditions annomontine failed to affect the behavior of the animals in the pentobarbital-induced hypnosis test and had no effects on locomotion and motor coordination. These results suggest that annomontine possesses anxiolytic-like effects which may be mediated at the level of the benzodiazepine binding site on the GABAA receptor.

References

Dr. José del Carmen Rejón-Orantes

Laboratorio Experimental de Farmacobiología
Facultad de Medicina, Universidad Autónoma de Chiapas

10 av. Sur y Calle Central S/N

Tuxtla Gutiérrez, Chiapas

C. P. 29040

México

Phone: + 52 96 16 12 49 24

Fax: + 52 96 22 29 21 17

Email: rejonjose@hotmail.com