Planta Med 2011; 77(11): 1168-1182
DOI: 10.1055/s-0030-1250736
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Plant-Based Antimicrobial Studies – Methods and Approaches to Study the Interaction between Natural Products

Sandy van Vuuren1 , Alvaro Viljoen2
  • 1Department of Pharmacy and Pharmacology, Faculty of Health Sciences, University of the Witwatersrand, Parktown, South Africa
  • 2Department of Pharmaceutical Sciences, Tshwane University of Technology, Pretoria, South Africa
Further Information

Publication History

received October 5, 2010 revised Dec. 9, 2010

accepted Dec. 16, 2010

Publication Date:
31 January 2011 (online)

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Abstract

The therapeutic value of synergistic interactions has been known since antiquity, and many different cultural healing systems still rely on this principle in the belief that combination therapy may enhance efficacy. This paper intends to provide an overview, from an antimicrobial perspective, on the research undertaken and interactive principles involved in pharmacognosy studies. Methods used to determine antimicrobial interactions include basic combination studies, the sum of the fractional inhibitory concentration index (ΣFIC), isobole interpretations, and death kinetic (time-kill) assays. The various interactions are discussed with reference to molecules, different plant parts or fractions, different plant species, and combinations with nonbotanical antimicrobial agents. It is recommended for future development in the field of phytosynergy that consideration should be given to the selection criteria for the two inhibitors. A more conservative approach should be adopted when classifying synergy. When examining interactions in plant-based studies, antagonistic interactions should not be ignored. Combinations involving more than two test samples should be examined where applicable, and very importantly, the mechanism of action of synergistic interactions should be given precedence. It is encouraging to observe the upsurge in papers exploring the complex interactions of medicinal plants, and undoubtedly this will become increasingly important in our continued quest to understand the mechanism of action of phytotherapy. The scientific validation of efficacious antimicrobial combinations could lead to patentable entities making research in the field of phytosynergy not only academically rewarding but also commercially relevant.

References

Prof. Sandy van Vuuren

Department of Pharmacy and Pharmacology
Faculty of Health Sciences
University of the Witwatersrand

7 York Road

Parktown 2193

South Africa

Phone: + 27 01 17 17 21 57

Fax: + 27 01 16 42 43 55

Email: Sandy.vanvuuren@wits.ac.za