Planta Med 2011; 77(15): 1707-1711
DOI: 10.1055/s-0030-1270993
Pharmakokinetic Investigations
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Determination of Corosolic Acid, a Natural Potential Anti-Diabetes Compound, in Rat Plasma by High-Performance Liquid Chromatography-Mass Spectrometry and its Application to Pharmacokinetic and Bioavailability Studies

Qingwang Liu1 , Di Zhao2 , Xijing Chen2 , Zhubo Li1 , Ning Li2 , Deen Han2 , Amer Talbi2 , Tarcisse Biwoele3
  • 1College of Pharmaceutical Sciences, Southwest University, Chongqing, P. R. China
  • 2Center of Drug Metabolisms and Pharmacokinetics, China Pharmaceutical University, Nanjing, Jiangsu, P. R. China
  • 3Department of Pharmacology, China Pharmaceutical University, Nanjing, Jiangsu, P. R. China
Further Information

Publication History

received Dec. 21, 2010 revised February 27, 2011

accepted March 21, 2011

Publication Date:
11 April 2011 (eFirst)


A simple, robust, and sensitive high-performance liquid chromatography/mass spectrometric method was developed for the determination of corosolic acid, a potential anti-diabetes substance, in rat plasma using glycyrrhetinic acid as the internal standard (IS). This method involved a liquid-liquid extraction with acetic ether and a subsequent analysis performed on an LC-MS system which contained an electrospray ionization interface. Chromatographic separation was performed using an ODS column, and the mobile phase was composed of methanol and 5 mmol/L ammonium acetate (88 : 12, v/v). Good linearity was observed over the concentration range of 20–10 000 ng/mL with a correlation coefficient (r2) ≥ 0.995. The method was proved to be accurate and reliable and was applied to a pharmacokinetic study in the rat following intragastric and intravenous administration of corosolic acid.


Prof. Dr. Zhubo Li

College of Pharmaceutical Sciences
Southwest University

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