Planta Med 2011; 77(15): 1756-1758
DOI: 10.1055/s-0030-1271083
Analytical Studies
Letters
© Georg Thieme Verlag KG Stuttgart · New York

Quantification of Cynaropicrin in Artichoke Leaf Extracts by 1H NMR Spectroscopy

Valerio Pieri1 , Hermann Stuppner1
  • 1Institute of Pharmacy/Pharmacognosy, Center for Molecular Biosciences Innsbruck, Leopold-Franzens University of Innsbruck, Innsbruck, Austria
Further Information

Publication History

received February 22, 2011 revised April 8, 2011

accepted April 13, 2011

Publication Date:
12 May 2011 (online)

Abstract

The quantification of cynaropicrin in artichoke leaf extracts (ALE) by means of quantitative 1H NMR was performed. The method relies on solid phase extraction for sample preparation and spectra acquisition in DMSO-d 6 containing anthracene as the internal standard. The obtained 1H NMR spectra revealed excellent signal purity for H-13b of cynaropicrin, which was thus chosen for quantification. Validation was performed in terms of selectivity, linearity, precision, accuracy, and robustness. The content of cynaropicrin in the analyzed samples showed considerable variation, ranging from non detectable to 1.6 %. The NMR approach does not rely on external calibration, which represents the major advantage compared to traditional approaches based on HPLC.

Supporting Information

References

Prof. Dr. Hermann Stuppner

Institute of Pharmacy/Pharmacognosy
Center for Molecular Biosciences Innsbruck
Leopold-Franzens University of Innsbruck

Innrain 52c, Josef-Moeller-Haus

6020 Innsbruck

Austria

Phone: +43 51 25 07 53 00

Fax: +43 51 25 07 29 39

Email: hermann.stuppner@uibk.ac.at