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Planta Med 2012; 78(4): 341-348
DOI: 10.1055/s-0031-1280472
Biological and Pharmacological Activity
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Paeoniae Radix Reduces PDGF-Stimulated Hepatic Stellate Cell Migration

Jong-Jen Kuo1 , 2 , Chih-Yang Wang1 , Ting-Fang Lee1 , Yi-Tsau Huang1 , 3 [*] , Yun-Lian Lin3 , 4 [*]
  • 1Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan
  • 2Center for Traditional Chinese Medicine, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan
  • 3National Research Institute of Chinese Medicine, Taipei, Taiwan
  • 4School of Pharmacy, National Taiwan University, Taipei, Taiwan
Further Information

Publication History

received October 21, 2011 revised Nov. 30, 2011

accepted Dec. 2, 2011

Publication Date:
13 January 2012 (online)

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Abstract

Hepatic stellate cells (HSCs) play a key role in the pathogenesis of liver fibrosis. In chronic liver injury, HSCs undergo transdifferentiation to an activated myofibroblastic phenotype and migrate to injured areas in response to chemotactic factors, producing extracellular matrix proteins such as collagen type I to repair the damage as well as overexpression of α-smooth muscle actin (α-SMA). Paeoniae Radix, the root of Paeonia lactiflora Pall, was investigated for PDGF-BB-induced HSC chemotaxis. Rat HSCs and LX-2, a human HSC cell line, were used for the in vitro experiments. Cell migration was analyzed by wound-healing and transwell assays. An ELISA and a Sircol collagen assay kit were used to detect the expressions of α-SMA and of collagen, respectively. Phosphorylations of mitogen-activated protein kinases, including ERK 1/2, p38, and JNK, were evaluated with immunoblotting. Results indicated that PDGF-BB increased migration as well as α-SMA and collagen expression in HSCs. Paeoniae Radix extracts and its active components, paeonol and 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG), inhibited PDGF-BB-induced HSC migration and α-SMA and collagen expressions in a concentration-dependent manner. The inhibitory effects were associated with downregulation of PDGF receptor-α, ERK, p38, and JNK activation. Both paeonol and PGG participate in HSC migration, but via differential mechanisms.

Key words

Paeonia lactiflora - Ranunculaceae - hepatic stellate cells - PDGF-BB - 1,2,3,4,6-penta-O-galloyl-β-D-glucose - paeonol - chemotaxis

Supporting Information

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1 Both authors contributed equally to this work and as corresponding authors.

Yun-Lian Lin

National Research Institute of Chinese Medicine

Taipei 112

Taiwan

Phone: +88 62 28 20 19 99 ext. 65 31

Fax: +88 62 28 25 07 43

Email: yllin@nricm.edu.tw

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