Planta Med 2011; 77 - PE50
DOI: 10.1055/s-0031-1282381

Antimicrobial Activities in Cultivated Origanum vulgare subsp. hirtum Populations of Different Origin

S Sancaktaroğlu 1, Ö Abacı 2, AB Tınmaz 3, AH Uztan 2
  • 1Iğdır University, Agriculture Faculty Field Crops Department,76000, Iğdır, Turkey
  • 2Ege University, Science Faculty, Microbiologi Department
  • 3Atatürk Horticultural Central Research Institute

Origanum L. genus belongs to Lamiaceae (Labiatae) family. It has antimicrobial activities on high rates. Especially Origanum vulgare L. has very efficient antimicrobial activities because of the high essential oil with its main components; thymol and carvacrol. Origanum vulgare L. subsp. hirtum (Link) Ietswaart, main components of the essential oil are carvacrol, thymol, γ-terpinene and p-cymene. This study was conducted in order to determine antimicrobial activities in cultivated Origanum vulgare subsp. hirtum populations of different origin, and to correlate harvest times with antimicrobial activity.

Species of bacteria tested were Staphylococcus aureus ATCC 6538, Staphylococcus epidermidis ATCC 12228, Streptococcus faecalis ATCC 29212, Bacillus cereus CCM 99, Salmonella typhimurium CCM 5445, Pseudomonas aeruginosa ATCC 27853.

Species of fungi tested were Candida albicans ATCC 10231, C. tropicalis RSSK 665, C. krusei ATCC 6258, C. parapsilosis ATCC 2219, C. dubliniensis CD 36, Aspergillus fumigatus NRRL 2999.

CLSI (The Clinical Laboratory Standards Institute') broth microdilution method was used for the determination of MIC (Minimum inhibition concentration). MIC was determined according to the CLSI M27-A2 for Candida species, CLSI M38-A microdilution for Aspergillus fumigatus and CLSI M2-A7 microdilution for bacteria.