Effect of Coffea canephora Aqueous Extract On Microbial Counts in Ex Vivo Oral Biofilms: A Case Study

Abstract

In the present study, the ex vivo antimicrobial effect of brewed coffee was tested on oral biofilms. For this, unsweetened and sweetened (10 % sucrose) brewed light-roasted Coffea canephora at 20 % was used in biofilms formed by non-stimulated saliva from three volunteers. After 30 min contact with unsweetened and sweetened brews, the average microorganism count in the biofilms reduced by 15.2 % and 12.4 %, respectively, with no statistical difference among them. We also observed a drop of microorganisms in the biofilms after treatment with sucrose solution at 5 % compared to control (saline) and to sucrose at 1 % and 3 %. In conclusion, Coffea canephora extract reduces the microbial count in oral biofilm, and our data suggest that sucrose concentration in coffee brew can influence its antimicrobial property against the referred biofilm.

Key words

Coffea canephora - Rubiaceae - antimicrobial activity - oral biofilm - chlorogenic acid - caffeine

• References

• 1 Jeon JG, Rosalen PL, Falsetta ML, Koo H. Natural products in caries research: current (limited) knowledge, challenges and future perspective. Caries Res 2011; 45: 243-263
• 2 Bowen WH, Koo H. Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms. Caries Res 2011; 45: 69-86
• 3 Arthur RA, Del Bel Cury AA, Graner RO, Rosalen PL, Vale GC, Paes Leme AF, Cury JA, Tabchoury CP. Genotypic and phenotypic analysis of S. mutans isolated from dental biofilms formed in vivo under high cariogenic conditions. Braz Dent J 2011; 22: 267-274
• 4 Alviano WS, Alviano DS, Diniz CG, Antoniolli AR, Alviano CS, Farias LM, Carvalho MA, Souza MM, Bolognese AM. In vitro antioxidant potential of medicinal plant extracts and their activities against oral bacteria based on Brazilian folk medicine. Arch Oral Biol 2008; 53: 545-552
• 5 Loesch WJ, Grossman NS. Periodontal disease as a specific, albeit chronic, infection: diagnosis and treatment. Clin Microbiol Rev 2001; 14: 727-752
• 6 Sofrata A, Lingström P, Baljoon M, Gustafsson A. The effect of miswak extract on plaque pH. An in vivo study. Caries Res 2007; 41: 451-454
• 7 Antonio AG, Moraes RS, Perrone D, Maia LC, Santos KRN, Iorio NLP, Farah A. Species, roasting degree and decaffeination influence the antibacterial activity of coffee against Streptococcus mutans . Food Chem 2010; 118: 782-788
• 8 Antonio AG, Iorio NLP, Pierro VSS, Candreva MS, Farah A, dos Santos KRN, Maia LC. Inhibitory properties of Coffea canephora extract against oral bacteria and its effect on demineralisation of deciduous teeth. Arch Oral Biol 2011; 56: 556-564
• 9 Matsumoto-Nakano M, Nagayama K, Kitagori H, Fujita K, Inagaki S, Takashima Y, Tamesada M, Kawabata S, Ooshima T. Inhibitory effects of Oenothera biennis (evening primrose) seed extract on Streptococcus mutans and S. mutans-induced dental caries in rats. Caries Res 2011; 45: 56-63
• 10 Almeida AAP, Farah A, Silva DAM, Nunan EA, Glória MB. Antibacterial activity of coffee extracts and selected coffee chemical compounds against enterobacteria. J Agric Food Chem 2006; 54: 8738-8743
• 11 Daglia M, Papetti A, Grisoli P, Aceti C, Spini V, Dacarro C, Gazzani G. Isolation, identification, and quantification of roasted coffee antibacterial compounds. J Agric Food Chem 2007; 55: 10208-10213
• 12 Ferrazzano GF, Amato I, Ingenito A, De Natale A, Pollio A. Anti-cariogenic effects of polyphenols from plant stimulant beverages (cocoa, coffee, tea). Fitoterapia 2009; 80: 255-262
• 13 Anila Namboodiripad PC, Kori S. Can coffee prevent caries?. J Conserv Dent 2009; 12: 17-21
• 14 Daglia M, Papetti A, Dacarro C, Gazzani G. Isolation of an antibacterial component from roasted coffee. J Pharm Biomed Anal 1998; 18: 219-225
• 15 Daglia M, Tarsi R, Papetti A, Grisoli P, Dacarro C, Pruzzo C, Gazzani G. Antiadhesive effect of green and roasted coffee on Streptococcus mutansʼ adhesive properties on saliva-coated hydroxyapatite beads. J Agric Food Chem 2002; 50: 1225-1229
• 16 Almeida AAP, Naghetini CC, Santos VR, Glória MB. In vitro antibacterial activity of coffee extracts on Streptococcus mutans . In: Propera, editors Proceedings of the 20th International Conference on Coffee Science – ASIC. Bangalore, India: ASIC; 2004: 242-248
• 17 Farah A, de Paulis T, Moreira DP, Trugo LC, Martin PR. Chlorogenic acids and lactones in regular and water-decaffeinated arabica coffees. J Agric Food Chem 2006; 54: 374-381
• 18 Perrone D, Donangelo CM, Farah A. Fast simultaneous analysis of caffeine, trigonelline, nicotinic acid and sucrose in green and roasted coffee by liquid chromatography – mass spectrometry. Food Chem 2008; 110: 1030-1035
• 19 Lima YBO, Cury JA. Ingestão de flúor por crianças pela água e dentifrício. Rev Saude Publica 2001; 35: 576-581
• 20 Martins C, Siqueira WL, de Oliveira E, Primo LS, Nicolau J. Salivary analysis of patients with chronic renal failure undergoing hemodialysis. Spec Care Dent 2006; 26: 205-208
• 21 Beighton D. The complex oral microflora of high-risk individuals and groups and its role in the caries process. Community Dent Oral Epidemiol 2005; 33: 248-255
• 22 Parsek M, Singh PK. Bacterial biofilms: an emerging link to disease pathogenesis. Annu Rev Microbiol 2003; 57: 677-701
• 23 Dogasaky C, Shindo T, Furuhata K, Fukuyama M. Identification of chemical structure of antibacterial components against Legionella pneumophila in a coffee beverage. Yakugaku Zasshi 2002; 122: 487-494
• 24 Stauder M, Papeti A, Daglia M, Vezzulli L, Gazzani G, Varaldo PE, Pruzzo C. Inhibitory activity by barley coffee components towards Streptococcus mutans biofilm. Curr Microbiol 2010; 61: 417-421
• 25 Simonssen T, Glantz PO, Edwardsson S. Effects of cations on the colloidal stability of some oral bacteria. Acta Odontol Scand 1988; 46: 83-87
• 26 Rupesh S, Winnier JJ, Nayak UA, Rao UA, Reddy NV. Comparative evaluation of the effects of an alum-containing mouthrinse and a saturated saline rinse on the salivary levels of Streptococcus mutans . J Soc Pedod Prev Dent 2010; 28: 138-144
• 27 Hayacibara MF, Ambrozano GM, Cury JA. Simultaneous release of fluoride and aluminum from dental materials in various immersion media. Oper Dent 2004; 29: 16-22
• 28 Kleber CJ, Putt MS. Aluminium and dental caries. A review of the literature. Clin Prev Dent 1984; 6: 14-25
• 29 Marinho VC, Higgins JP, Logan S, Sheiham A. Topical fluoride (toothpastes, mouthrinses, gels or varnishes) for preventing dental caries in children and adolescents. Cochrane Database Syst Rev 2003; (4) CD002782
• 30 Marinho VC, Higgins JP, Sheiham A, Logan S. Combinations of topical fluoride (toothpastes, mouthrinses, gels, varnishes) versus single topical fluoride for preventing dental caries in children and adolescents. Cochrane Database Syst Rev 2004; (1) CD002781
• 31 Tenuta LMA, Cury JA. Fluoride: its role in dentistry. Braz Oral Res 2010; 24: 9-17
• 32 Marquis RE, Clock AS, Mota-Meira M. Fluoride and organic weak acids as modulators of microbial physiology. FEMS Microbiol Rev 2003; 26: 493-510
• 33 Emilson CG. Potential efficacy of chlorhexidine against mutans streptococci and human dental caries. J Dent Res 1994; 73: 682-691
• 34 Bowen WH. Do we need to be concerned about dental caries in the coming millennium?. Crit Rev Oral Biol Med 2002; 13: 126-131
• 35 Quivey Jr. RG, Kuhnert WL, Hahn K. Adaptation of oral streptococci to low pH. Adv Microb Physiol 2000; 42: 239-274
• 36 Vale GC, Tabchoury CP, Arthur RA, Del Bel Cury AA, Paes Leme AF, Cury JA. Temporal relationship between sucrose-associated changes in dental biofilm composition and enamel demineralization. Caries Res 2007; 41: 406-412
• 37 Lemos JAC, Abranches J, Burne RA. Responses of cariogenic streptococci to environmental stresses. Curr Issues Mol Biol 2005; 7: 95-108