Anti-diabetic properties of non-polar Toona sinensis Roem extract prepared by supercritical-CO2 fluid

YH Tsai; TJ Hsieh; MC Liao; PJ Lien; CC Sun; FR Chang; YC Wu

doi:10.1055/s-0032-1320563

Planta Medica, Inhaltsverzeichnis

Anti-diabetic properties of non-polar Toona sinensis Roem extract prepared by supercritical-CO2 fluid

YH Tsai ¹^,³, TJ Hsieh ², MC Liao ², PJ Lien ⁴, CC Sun ⁴, FR Chang ¹^,³, YC Wu ⁵

¹Department of Medical Genetics, College of Medicine, Kaohsiung Medical University, 80708, Taiwan
²Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, 80708, Taiwan
³Research and Development Center of Chinese Herbal Medicines and New Drugs, Kaohsiung Medical University, 80708, Taiwan
⁴Metal Industries Research and Development Centre, 811, Taiwan
⁵College of Chinese Medicine, School of Chinese Medicine, China Medical University, 40402, Taiwan

Abstract

Toona sinensis Roem (T. sinensis) leaves have been used as a nutritious vegetable and been suggested for medical applications; however, the reported bioactive compounds of T. sinensis leaves are, so far, from high to mid-high polar extracts. Our aims in this study were to reveal the non-polar constituents of the T. sinensis leave extract that were prepared by a method of using a supercritical-CO₂ fluid and to investigate the anti-diabetic potential of this extract. Through a GC/MS analysis, we revealed 24 major components of the non-polar T. sinensis leave extract. The non-polar T. sinensis leave extract showed to prevent the progression of diabetes and hepatosteatosis, the rise of triglycerol levels and the decrease of adiponectin levels in the type 2 diabetic mice. Our results suggest that the non-polar extract of T. sinensis leaves prepared using the supercritical-CO₂ fluid may contain effective constituents to prevent type 2 diabetes.