Edible Passiflora (banana passion) fruits: a source of bioactive C-glycoside flavonoids obtained by HSCCC and HPLC-DAD-ESI/MS/MS

Tumbo fruits are edible fruits consumed raw and widely used in Ecuador, Peru and northern Chile to prepare an aromatic and acidic fruit juice. We have analyzed ethanolic extracts from peel and pulp of fruits of this plant by HPLC- DAD and HPLC-MS, and quantified their antioxidant as well as their flavonoid and phenolic content by spectroscopic methods. The fast fingerprint analysis allowed the detection of a family of twenty eight C-glycoside and three O-glycoside flavonol derivatives which were characterized by UV and ESI-MS-MS. Several C-glycosides are structurally related to the new orientin derivative 4'-methoxy-luteolin-8-C-(6”acetyl)-β-D-glucopyranoside, 1 which was isolated from the peel along with other derivatives (2–5) by HSCCC and fully elucidated by spectroscopic methods. The pulp of the fruits showed good antioxidant activity, but the peel presented the highest content of flavonoids, which is related to the highest antioxidant and antiradical activity.