Planta Med 2013; 79(05): 327-329
DOI: 10.1055/s-0032-1328259
Biological and Pharmacological Activity
Letters
Georg Thieme Verlag KG Stuttgart · New York

Sarothrin from Alkanna orientalis Is an Antimicrobial Agent and Efflux Pump Inhibitor

Jessica R. Bame
1  Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, Greensboro, NC, USA
,
Tyler N. Graf
1  Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, Greensboro, NC, USA
,
Hiyas A. Junio
1  Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, Greensboro, NC, USA
,
R. Owen Bussey III
1  Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, Greensboro, NC, USA
,
Scott A. Jarmusch
,
Tamam El-Elimat
1  Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, Greensboro, NC, USA
,
Joseph O. Falkinham III
2  Department of Biological Sciences, Virginia Tech., Blacksburg, VA, USA
,
Nicholas H. Oberlies
1  Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, Greensboro, NC, USA
,
Richard A. Cech
3  Horizon Herbs, LLC, Williams, OR, USA
,
Nadja B. Cech
1  Department of Chemistry and Biochemistry, The University of North Carolina Greensboro, Greensboro, NC, USA
› Author Affiliations
Further Information

Publication History

received 30 November 2012
revised 15 January 2013

accepted 21 January 2013

Publication Date:
06 March 2013 (online)

Abstract

An Alkanna orientalis leaf and flower extract inhibited the growth of Staphylococcus aureus, a pathogen that causes an estimated 478 000 hospitalizations in the US annually. Bioassay-guided fractionation of A. orientalis resulted in isolation of the flavonoid sarothrin (5,7,4′-trihydroxy-3,6,8-trimethoxyflavone), which inhibited the growth of Mycobacterium smegmatis (MIC 75 µM) and S. aureus (MIC > 800 µM), and possessed efflux pump inhibitory activity. This is the first report of antimicrobial or efflux pump inhibitory activity of sarothrin, and of its presence in A. orientalis. Our findings suggest that the effectiveness of A. orientalis extracts is due to a combination of multiple constituents, including sarothrin.

Supporting Information