Salvia officinalis for Hot Flushes: Towards Determination of
                    Mechanism of Activity and Active Principles

Sinikka Rahte; Richard Evans; Philippe J. Eugster; Laurence Marcourt; Jean-Luc Wolfender; Andreas Kortenkamp; Deniz Tasdemir

doi:10.1055/s-0032-1328552

Planta Medica, Inhaltsverzeichnis

Planta Med 2013; 79(09): 753-760
DOI: 10.1055/s-0032-1328552

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Salvia officinalis for Hot Flushes: Towards Determination of Mechanism of Activity and Active Principles

Authors

Sinikka Rahte

¹Centre for Pharmacognosy and Phytotherapy, School of Pharmacy, University of London, London, United Kingdom
Richard Evans

²Centre for Toxicology, School of Pharmacy, University of London, London, United Kingdom

³Current address: Institute for the Environment, Brunel University, Uxbridge, United Kingdom
Philippe J. Eugster

⁴School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland
Laurence Marcourt

⁴School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland
Jean-Luc Wolfender

⁴School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland
Andreas Kortenkamp

²Centre for Toxicology, School of Pharmacy, University of London, London, United Kingdom

³Current address: Institute for the Environment, Brunel University, Uxbridge, United Kingdom
Deniz Tasdemir

¹Centre for Pharmacognosy and Phytotherapy, School of Pharmacy, University of London, London, United Kingdom

⁵Current address: School of Chemistry, National University of Ireland, Galway, Ireland

Abstract

Herbal medicinal products are commonly used in alternative treatment of menopausal hot flushes. In a recent clinical study, Salvia officinalis tincture was found to reduce hot flush frequency and intensity. The aim of the current study was the investigation of the mechanism(s) responsible for the anti-hot flush activity of S. officinalis and determination of its active principle(s). The 66 % ethanolic tincture, as well as the n-hexane, CHCl₃, and aqueous ethanolic subextracts obtained from the tincture were studied in vitro for two of the most relevant activities, estrogenicity and selective serotonin reuptake inhibition. Because of an increased risk of menopausal women to suffer from Alzheimerʼs disease, an in vitro acetylcholinesterase inhibition assay was also employed. No activity was observed in the selective serotonin reuptake inhibition or the acetylcholinesterase inhibition assays at the highest test concentrations. The tincture showed no estrogenic effects whereas the aqueous ethanolic subextract exhibited estrogenicity in the ERLUX assay with an EC₅₀ value of 64 µg/mL. Estrogenic activity-guided fractionation of the aqueous ethanolic subextract by a combination of reverse-phase vacuum liquid chromatography and gel chromatography identified luteolin-7-O-glucuronide (EC₅₀ 129 µg/mL) as the active component of the vacuum liquid chromatography fraction 4 (EC₅₀ 69 µg/mL). Luteolin-7-O-glucoside was identified as the putative estrogenic principle of the most potent minor fraction (7.6.7.6, EC₅₀ 0.7 µg/mL) obtained from the initial vacuum liquid chromatography fraction 7 (EC₅₀ 3 µg/mL). This study suggests the involvement of common and ubiquitous estrogenic flavonoids in the anti-hot flush effect of Salvia officinalis, a safe and commonly used herbal medicinal product during the menopause.

Key words

estrogenicity - hot flush - luteolin-7-O-glucoside - menopause - Salvia officinalis - Lamiaceae

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Referenzen

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