Simultaneous Quantification of Fourteen Major Compounds in Traditional Herbal Medicine, Samchulgunbitang by HPLC-DAD
A reliable and accurate high-performance liquid chromatograph coupled with a diode array detector method was established and validated for the determination of the fourteen compounds, ginsenoside Rg1, ginsenoside Rg3, atractylenolide I, atractylenolide III, paeoniflorin, albiflorin, magnolol, hesperidin, naringin, chlorogenic acid, hyperoside, poncirin, glycyrrhizin, 6-gingerol in traditional herbal medicine, Samchulgunbitang. The HPLC-DAD separation was performed on the SHISHEDO C18 column (5 µm, 4.6 I.D.×250 mm) with gradient system using a mobile phase consisting of 0.1% TFA (trifluoroacetic acid) water (A) and acetonitrile (B). The HPL-DAD method was validated for linearity, inter-day and intra-day precision, accuracy. All compounds showed good linearity (R2> 0.9996) within the test ranges. The intra-day precision ranged from 0.60% to 2.88%, and the inter-day precision ranged from 0.74% to 2.89%. The recovery rates for all analytes were 90.40 – 109.94%. Eleven commercial samples were successfully analyzed using the developed HPLC-DAD method. The results indicated that this method will be very helpful for the quality control of Samchulgunbitang. Acknowledgements: This research was supported by a grant [K12050] from the Korea Institute of Oriental Medicine.