Simultaneous Quantification of Fourteen Major Compounds in Traditional Herbal Medicine, Samchulgunbitang by HPLC-DAD

A reliable and accurate high-performance liquid chromatograph coupled with a diode array detector method was established and validated for the determination of the fourteen compounds, ginsenoside Rg₁, ginsenoside Rg₃, atractylenolide I, atractylenolide III, paeoniflorin, albiflorin, magnolol, hesperidin, naringin, chlorogenic acid, hyperoside, poncirin, glycyrrhizin, 6-gingerol in traditional herbal medicine, Samchulgunbitang. The HPLC-DAD separation was performed on the SHISHEDO C18 column (5 µm, 4.6 I.D.×250 mm) with gradient system using a mobile phase consisting of 0.1% TFA (trifluoroacetic acid) water (A) and acetonitrile (B). The HPL-DAD method was validated for linearity, inter-day and intra-day precision, accuracy. All compounds showed good linearity (R²> 0.9996) within the test ranges. The intra-day precision ranged from 0.60% to 2.88%, and the inter-day precision ranged from 0.74% to 2.89%. The recovery rates for all analytes were 90.40 – 109.94%. Eleven commercial samples were successfully analyzed using the developed HPLC-DAD method. The results indicated that this method will be very helpful for the quality control of Samchulgunbitang. Acknowledgements: This research was supported by a grant [K12050] from the Korea Institute of Oriental Medicine.