Planta Med 2013; 79(14): 1319-1323
DOI: 10.1055/s-0033-1350649
Biological and Pharmacological Activity
Letters
Georg Thieme Verlag KG Stuttgart · New York

Antioxidative and Anti-inflammatory Activities of Paeoniflorin and Oxypaeoniflora on AGEs-Induced Mesangial Cell Damage

Ming-hua Zhang
1   Key Laboratory of New Drug Delivery Systems of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing, P. R. China
2   Department of Pharmaceutics, Jiangsu University, Jiangsu, Zhenjiang, P. R. China
,
Liang Feng
1   Key Laboratory of New Drug Delivery Systems of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing, P. R. China
,
Mao-mao Zhu
1   Key Laboratory of New Drug Delivery Systems of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing, P. R. China
3   Nanjing Institute of Supervision & Testing on Product Quality, Jiangsu, Nanjing, P. R. China
,
Jun-fei Gu
1   Key Laboratory of New Drug Delivery Systems of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing, P. R. China
4   College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu, Nanjing, P. R. China
,
Chan Wu
4   College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu, Nanjing, P. R. China
,
Xiao-bin Jia
1   Key Laboratory of New Drug Delivery Systems of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing, P. R. China
› Author Affiliations
Further Information

Publication History

received 01 May 2013
revised 19 June 2013

accepted 29 June 2013

Publication Date:
23 July 2013 (online)

Abstract

Paeonia suffruticosa, an important traditional herbal medicine, has been reported to prevent the pathogenesis of diabetic nephropathy through modulating advanced glycation end products-induced inflammatory and oxidative stress responses. However, little was known about the protective effect of the two major compounds in P. suffruticosa, paeoniflorin and oxypaeoniflora, on advanced glycation end products-induced mesangial cell damage. In the present study, we investigated the protective activities of paeoniflorin and oxypaeoniflora on advanced glycation end product-induced oxidative stress and inflammation in mesangial cells HBZY-1. The IC50 values of paeoniflorin and oxypaeoniflora for inhibiting 2,2′-azinobis-(3-thylbenzothiazoline-6-sulfonic acid) formation were 4.197 × 10−4 M and 1.002 × 10−4 M, respectively. The pretreatment with paeoniflorin and oxypaeoniflora (10−8–10−4 M) significantly increased advanced glycation end product-induced glutathione peroxidase and catalase activities. In the coculture system of HBZY-1 and macrophages, paeoniflorin and oxypaeoniflora could inhibit remarkably the migration of macrophages. Furthermore, paeniflorin and oxypaeniflora attenuated markedly advanced glycation end products-induced inflammation cytokines interleukin-6 and monocyte chemoattractant protein-1 levels in ELISA and western blot analysis in a dose-dependent manner. Taken together, our data provided the reliable evidence that paeniflorin and oxypaeniflora were able to attenuate advanced glycation end products-induced oxidative damage and inflammation in mesangial cells. Paeniflorin and oxypaeniflora might therefore have a beneficial effect in the treatment of diabetic nephropathy.

Supporting Information

 
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