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Planta Med 2013; 79(16): 1516-1524
DOI: 10.1055/s-0033-1350809
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

The Mechanisms of In Vitro Cytotoxicity of Mountain Tea, Sideritis scardica, against the C6 Glioma Cell Line

Ivica Jeremic
1   Institute of Biochemistry, School of Medicine, University of Belgrade, Belgrade, Serbia
2   Institute of Rheumatology, Belgrade, Serbia
,
Vanja Tadic
3   Institute for Medicinal Plant Research “Dr Josif Pančić”, Belgrade, Serbia
,
Andjelka Isakovic
1   Institute of Biochemistry, School of Medicine, University of Belgrade, Belgrade, Serbia
,
Vladimir Trajkovic
4   Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Belgrade, Serbia
,
Ivanka Markovic
1   Institute of Biochemistry, School of Medicine, University of Belgrade, Belgrade, Serbia
,
Zoran Redzic
5   Department of Physiology, Faculty of Medicine, Kuwait University, Safat, Kuwait
,
Aleksandra Isakovic
1   Institute of Biochemistry, School of Medicine, University of Belgrade, Belgrade, Serbia
› Author Affiliations
Further Information

Publication History

received 19 March 2013
revised 22 July 2013

accepted 07 August 2013

Publication Date:
26 September 2013 (online)

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Abstract

Sideritis scardica (mountain tea) is an endemic plant on the Balkan Peninsula traditionally used for treating different conditions, mainly of inflammatory nature. This study was aimed to examine the cytotoxic activity of different S. scardica extracts against the rat glioma C6 line and rat astrocytes in primary culture. The obtained data revealed that diethyl ether (extract 2) and ethyl acetate (extract 3) extracts of S. scardica exerted a cytotoxic effect on C6 rat glioma cells. Diethyl ether extract induced an increase in reactive oxygen species production, leading to apoptotic and autophagic cell death. Ethyl acetate extract induced G2 M cell cycle arrest and autophagy. None of the tested extracts was cytotoxic to rat astrocytes in primary culture. Cytotoxic effects of S. scardica extracts were, at least in part, mediated by their flavonoid constituents apigenin and luteolin that, when applied alone, induced cell cycle arrest, apoptosis, and autophagy.

Key words

Sideritis scardica - Lamiaceae - apigenin - luteolin - C6 glioma cells - autophagy
 

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