The Mechanisms of In Vitro Cytotoxicity of Mountain Tea,
                        Sideritis scardica, against the C6 Glioma Cell Line

Ivica Jeremic; Vanja Tadic; Andjelka Isakovic; Vladimir Trajkovic; Ivanka Markovic; Zoran Redzic; Aleksandra Isakovic

doi:10.1055/s-0033-1350809

Planta Medica, Table of Contents

Planta Med 2013; 79(16): 1516-1524
DOI: 10.1055/s-0033-1350809

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

The Mechanisms of In Vitro Cytotoxicity of Mountain Tea, Sideritis scardica, against the C6 Glioma Cell Line

Ivica Jeremic

¹Institute of Biochemistry, School of Medicine, University of Belgrade, Belgrade, Serbia

²Institute of Rheumatology, Belgrade, Serbia

,

Vanja Tadic

³Institute for Medicinal Plant Research “Dr Josif Pančić”, Belgrade, Serbia

,

Andjelka Isakovic

¹Institute of Biochemistry, School of Medicine, University of Belgrade, Belgrade, Serbia

,

Vladimir Trajkovic

⁴Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Belgrade, Serbia

,

Ivanka Markovic

¹Institute of Biochemistry, School of Medicine, University of Belgrade, Belgrade, Serbia

,

Zoran Redzic

⁵Department of Physiology, Faculty of Medicine, Kuwait University, Safat, Kuwait

,

Aleksandra Isakovic

¹Institute of Biochemistry, School of Medicine, University of Belgrade, Belgrade, Serbia

› Author Affiliations

Abstract

Sideritis scardica (mountain tea) is an endemic plant on the Balkan Peninsula traditionally used for treating different conditions, mainly of inflammatory nature. This study was aimed to examine the cytotoxic activity of different S. scardica extracts against the rat glioma C6 line and rat astrocytes in primary culture. The obtained data revealed that diethyl ether (extract 2) and ethyl acetate (extract 3) extracts of S. scardica exerted a cytotoxic effect on C6 rat glioma cells. Diethyl ether extract induced an increase in reactive oxygen species production, leading to apoptotic and autophagic cell death. Ethyl acetate extract induced G₂ M cell cycle arrest and autophagy. None of the tested extracts was cytotoxic to rat astrocytes in primary culture. Cytotoxic effects of S. scardica extracts were, at least in part, mediated by their flavonoid constituents apigenin and luteolin that, when applied alone, induced cell cycle arrest, apoptosis, and autophagy.

Key words

Sideritis scardica - Lamiaceae - apigenin - luteolin - C6 glioma cells - autophagy

Full Text

References

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