Abstract
Cyanidin and delphinidin are the main phenolic antioxidants in the grape
(Vitis vinifera). The aim of this study was to investigate the
in vitro and in vivo inhibitory effects of delphinidin and
cyanidin chloride in the free and liposomal forms on the albumin glycation
reaction. Delphinidin and cyanidin chlorides were encapsulated in the
liposomes using an extrusion method. The rate of albumin glycation was
evaluated using the ELISA method. Finally, in vivo anti-glycation of
delphinidin and cyanidin chloride in the free and liposomal forms in
diabetic mice was investigated. The encapsulation efficacies of delphinidin
and cyanidin chloride in the liposomes were 89.05 % ± 0.18 and
85.00 % ± 0.15, respectively. In vitro treatment with 100 mg/mL
delphinidin and cyanidin chloride in free forms could reduce the rate of
albumin glycation to 30.50 ± 3.46 and 46.00 ± 2.50 %, respectively. Under
identical conditions, the delphinidin and cyanidin chloride-loaded liposomes
could reduce the rate of albumin glycation to 8.50 ± 2.10 and
14.60 ± 3.60 %, respectively. In vivo testing showed that
anti-glycation activity of delphinidin and cyanidin in loaded forms was
higher than in free forms. The daily administration of 100 mg/kg delphinidin
chloride-loaded liposomes to diabetic mice at eight weeks could decrease the
rate of albumin and HbA1c glycation to 46.35 ± 1.20 and 3.60 ± 0.25 %,
respectively. Moreover, under identical conditions, the loaded liposomes
with cyanidin chloride could decrease the rate of albumin and HbA1c
glycation to 55.56 ± 1.32 and 4.95 ± 0.20 %, respectively. The findings
showed that delphinidin and cyanidin chloride, in particular in the
liposomal forms, could be used for treatment of diabetes mellitus
complications.
Key words
Vitis vinifera
- Vitaceae - albumin glycation - cyanidin chloride - delphinidin chloride - liposome
