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Eur J Pediatr Surg 2015; 25(03): 242-249
DOI: 10.1055/s-0034-1371714
Original Article
Georg Thieme Verlag KG Stuttgart · New York

Histopathological Changes at Colonic Anastomotic Site after Ischemia Reperfusion Injury: Role of Aminoguanidine in Experimental Model

Shailesh Solanki
1   Department of Pediatric Surgery, All India Institute of Medical Sciences, New Delhi, India
,
M. Srinivas
1   Department of Pediatric Surgery, All India Institute of Medical Sciences, New Delhi, India
,
Anand Sinha
1   Department of Pediatric Surgery, All India Institute of Medical Sciences, New Delhi, India
,
Deepak Mittal
1   Department of Pediatric Surgery, All India Institute of Medical Sciences, New Delhi, India
,
Saumyaranjan Mallick
2   Department of Pathology, All India Institute of Medical Sciences, New Delhi, India
,
Sandeep Agarwala
1   Department of Pediatric Surgery, All India Institute of Medical Sciences, New Delhi, India
,
V. Bhatnagar
1   Department of Pediatric Surgery, All India Institute of Medical Sciences, New Delhi, India
› Institutsangaben
Weitere Informationen

Publikationsverlauf

18. August 2013

11. Januar 2014

Publikationsdatum:
28. März 2014 (online)

    Lizenzen und Reprints

Abstract

Background Inducible nitric oxide synthase (iNOS) has a significant role in ischemia reperfusion (I-R) injury. I-R injury impairs the healing at the intestinal anastomotic site. This study was designed to assess the role of aminoguanidine (AG, a selective inhibitor of iNOS), in healing at the colonic anastomotic site after intestinal I-R injury in rats.

Methods Female Wistar rats (n = 60) were divided into three groups. Group I (n = 15): sham operation, Group II (n = 15): I-R injury and anastomosis, and Group III (n = 30): I-R injury + anastomosis + AG-50 mg/kg. On the 7th postoperative day, relaparotomy was done and 4 cm of the colon with an intact area of the anastomosis was resected. Bursting pressure and histology at the anastomotic site were assessed.

Results The bursting pressure was significantly higher in Group III. In addition, bridging parameters (i.e., mucosal continuity, muscular continuity, re-epithelization, and granulation tissue), collagen pattern, and collagen density were significantly better in Group III. While the polymorphonuclear density was higher in Group II, suggestive of delayed healing.

Conclusion AG, by decreasing inflammation and increasing collagen content in an organized pattern, helped in preventing I-R injury at the site of colonic anastomosis in rats.

Keywords

ischemia reperfusion injury - nitric oxide synthase - aminoguanidine - intestinal anastomosis healing
 

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