Planta Med 2015; 81 - PA17
DOI: 10.1055/s-0035-1545146

Simultaneous quantification of flavonoids Homoeriodictyol and Persicogenin in the methanol extract of aerial parts of two different species of genus Rhus by validated HPTLC method

P Alam 1, MS Ahmad 1, TA Wani 2, S Zargar 3, S Singh 4, NA Siddiqui 1
  • 1Dept. of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
  • 2Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
  • 3Department of Biochemistry, College of Science, King Saud University, PO Box 22452, Riyadh 11211, Saudi Arabia
  • 4Department of Pharmacy, I.E.T., M.J.P.Rohilkhand University, Bareilly-243006, U.P., India

A simple, sensitive HPTLC-densitometric method was developed for simultaneous quantification of two flavonoid compounds, persicogenin and homoeriodictyol, in the methanol extracts of aerial parts of two species (Rhus retinorrohea and Rhus tripartita) of the genus Rhus grown in the Kingdom of Saudi Arabia. Chromatography was performed on glass-backed silica gel 60 F254 HPTLC plates using toluene: ethyl acetate: methanol (8:2:0.5, v/v/v) as the mobile phase. Scanning and quantification were carried out at 293nm. The system was found to give compact spots for homoeriodictyol and persicogenin at Rf = 0.30 ± 0.01 and 0.48 ± 0.01, respectively. The linearity ranges for homoeriodictyol and persicogenin were found to be the same (100 – 800 ng/spot) with correlation coefficients (r2 values) of 0.9989 and 0.9983, respectively. The LODs for homoeriodictyol and persicogenin were determined to be 26 and 31 ng band-1, respectively while the LOQ was found to be 77 and 92 ng band-1, respectively. Homoeriodictyol (7.06%) and persicogenin (2.33%) were only found in Rhus retinorrohea. The developed method was found to be accurate and precise, hence it can be used as an important tool to assure the therapeutic dose of homoeriodictyol and persicogenin in herbal formulations as well as for standardization and quality control of bulk drugs.