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Planta Med 2015; 81(10): 784-790
DOI: 10.1055/s-0035-1546080
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Antioxidative Activity of Flavonoids from Abrus cantoniensis against Ethanol-Induced Gastric Ulcer in Mice

Hui Li   *
1   Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China
,
Zi-Jing Song   *
1   Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China
,
Yan-Ping Dai
1   Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China
,
Su-Li Zhang
1   Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China
,
Xin He
2   School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, JS, China
,
Chang-Run Guo
1   Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China
,
Wen-Jun Zhang
1   Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China
,
Jiao-Ying Wang
1   Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China
,
Chun-Feng Zhang
1   Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China
3   Tang Center of Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL, USA
,
Chong-Zhi Wang
3   Tang Center of Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL, USA
,
Chun-Su Yuan
3   Tang Center of Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL, USA
› Author Affiliations
Further Information

Publication History

received 25 November 2014
revised 24 March 2015

accepted 19 April 2015

Publication Date:
03 June 2015 (online)

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Abstract

The present study investigated the flavonoids from Abrus cantoniensis against ethanol-induced gastric ulcers in mice. The flavonoids from A. cantoniensis were extracted with ethanol and purified by macroporous resin and polyamide. The 2,2-diphenyl-1-picrylhydrazyl assay was used to measure the antioxidative activities in vitro. The ethanol-induced ulcer mouse model was used to evaluate the gastroprotective activities of the flavonoids from A. cantoniensis. In addition, a method was established to ensure accuracy for animal ulcer evaluation. The flavonoids from A. cantoniensis showed a strong free radical scavenging capacity with an IC50 of 43.83 µg/mL in the 2,2-diphenyl-1-picrylhydrazyl assay. At doses between 28.16–112.67 mg/kg, the flavonoids conspicuously reduced the ulcer index in ethanol-induced mice (p < 0.001). Significant differences were found in the levels of superoxide dismutase, catalase, glutathione, and myeloperoxidase in the stomach tissues between the flavonoids from the A. cantoniensis groups and the ethanol control group. The gastroprotective effect of the flavonoids from A. cantoniensis could be due to its antioxidative activity of the defensive mechanism. The data revealed that the flavonoids from A. cantoniensis could be a potential therapeutic agent for gastric ulcer prevention and treatment.

Key words

Abrus cantoniensis - Leguminosae - flavonoid - antioxidant - gastroprotection - Image Pro-Plus

* These two authors contributed equally to this work.


Supporting Information

 

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