Planta Med 2015; 81 - PM_176
DOI: 10.1055/s-0035-1565553

Studies on angiogenic potential of Rubia cordifolia, Mimosa pudica and Hemidesmus indicus by chick embryo chorioallantoic membrane as a model system

AK Vora 1, M Gore 2, N Thakur 1, A Siddhanti 1, D Gatne 1, Y Kachwala 1, M Bhaskar 2
  • 1Department of Pharmaceutical Chemistry, SPP-School of Pharmacy &Technology Management, SVKM's NMIMS, Mumbai, India
  • 2Department of Pharmacology, SPP-School of Pharmacy & Technology Management, SVKM's NMIMS, Mumbai, India

In wound healing process, angiogenesis plays an important physiological role by restoring blood flow to tissues after injury.

The chorioallantoic membrane (CAM) is a vascular extra-embryonic membrane found in eggs of some amniotes, such as birds, and is formed on day 4 of incubation. It is formed by the fusion of the allantois and chorion. The CAM model has been widely used to screen and evaluate the wound healing potential of various compounds. Rubia cordifolia (RC), Mimosa pudica (MP) and Hemidesmus indicus (HI) are reported for their wound healing property. But till date no reports were available for their mechanistic role in wound healing via angiogenesis.

The aqueous extracts and fractions of RC, MP and HI were tested for angiogenesis using chick embryo chorioallantoic membrane as a model system.

Result:

RC: The percentage increase in number of blood vessels observed were 48.81% with 0.1 µg/mL, 32.65% with 1 µg/mL. The concentration 10 µg/mL was found to be toxic to CAM.

Hi: The percentage increase in number of blood vessels observed were 30.25% with 1 µg/mL, 41.45% with 10 µg/mL and 61.37% with 100 µg/mL.

MP: The percentage increase in number of blood vessels observed were 48.21% with 1 µg/mL, 68.32% with 10 µg/mL and 70.43% with 100 µg/mL.

Rubia cordifolia, Mimosa pudica and Hemidesmus indicus promoted angiogenesis in CAM model. The angiogenesis potential of the plants extracts could be contributed to the alkaloids and flavonoids which are reported to be responsible for wound healing. Hence, we propose ''angiogenesis'' as a probable mechanism towards wound healing properties of these extracts. Angiogenesis, the increased blood vessel growth can promote both the extent and direction of fibroplasia. Neovascularization, therefore, would be contributing significantly to wound healing activity of Rubia cordifolia, Mimosa pudica and Hemidesmus indicus. They may serve as a good source for isolating lead compounds for wound healing property.