Planta Med 2015; 81 - PW_79
DOI: 10.1055/s-0035-1565703

Anti-inflammatory and antioxidant activity of white lupin (Lupinus albus) aerial parts

F Sibul 1, D Orcic 1, J Nadjpal 1, Z Mrkonjic 1, M Franciskovic 1, T Majkic 1, K Bekvalac 1
  • 1Department for Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia

Lupinus albus L. (white lupin), is a member of the family Fabaceae, known for high contents of secondary metabolites with potential biological activities. In this paper, antioxidant and anti-inflammatory potential of aerial parts were investigated, since little is known about its aforementioned properties.

80% MeOH herb extract was prepared by maceration. Anti-inflammatory potential was examined in human platelets, using method based on determination of inhibition ability of eicosanoid production [1]. For examination of antioxidative capacity, spectrophotometric methods were used. DPPH·neutralization and NO·scavenging ability was determinated, and reduction potential was examined using the FRAP assay.

Anti-inflammatory activity of is expressed in IC 50 values of eicosanoid production inhibition: 12-HETE (12-hydroxyeicosatetraenoic acid) 7.13 mg/mL, 12-HHT (12(S)-hydroxyheptadeca-5Z,8E,10E-trienoic acid) 5.66 mg/mL and TXB2 (thromboxane B2) 4.94 mg/mL, while for PGE2 IC50 value was not reached. IC50 value for DPPH·neutralization is 100.3 µg/mL, and for NO·scavenging 1.95 mg/mL. Reduction potential is 15.0 mg vitamin C equivalents per g of dry weight. High reduction potential and free radical scavenging ability makes the white lupin herb extract a good source of antioxidant agents, while it has shown only moderate anti-inflammatory activity.

Acknowledgement: This work was supported by a research grant from the Ministry of Education and Science, Republic of Serbia (Grant No. 172058).

References:

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