Determinatin of proscillaridin in Drimia maritima from two provinces of Iran

Drimia maritima (L.) is an endemic plant of Iran that have been used for varies disease such as dropsy, asthma and cancer since ancient time [1]. Bufadienolides are the main constituents of this plant. Proscillaridin is one of the bufadienolides that possess important biological activities such as antitumor, and T- cell suppressive effects [2, 3]. This compound is determined in D. maritima grows in two distinct regions of Iran. Powder of dried bulbs (0.25 g) was extracted with methanol (10 ml) via ultrasonic method (60 min, 40 °C, three times). A validated high performance liquid chromatography (HPLC) method was approved for proscillandin determination in our previous study [4]. The chromatographic separation was carried out on a reversed phase ACE C18 with eluting at flow rate of 1 mL/min using a gradient with methanol-water for 50 min. Proscillaridin amount of D. maritima from Fars and Hamedan provinces determined as 4.1 and 1.5 (µg/mL) respectively. So utilization of samples from Fars provinces may be more beneficial for preparing herbal formulations consisting proscillaridin.

References:

[1] Bozorgi M, Amin GH, Shekarchi M, Rahimi R. Medicinal Plants of the Genus Drimia: a Review on Traditional Uses, Phytochemistry, Pharmacology and Toxicology. J Tradit Chin Med In press

[2] Bielawski K, Winnicka K, Bielawska A. Inhibition of DNA topoisomerases I and II, and growth inhibition of breast cancer MCF-7 cells by ouabain, digoxin and proscillaridin A. Biol Pharm Bull 2006; 29: 1493 – 1497

[3] Terness P, Navolan D, Dufter C, Kopp B, Opelz G. The T-cell suppressive effect of bufadienolides: structural requirements for their immunoregulatory activity. IntImmunopharmacol 2001; 1: 119 – 134

[4] Kasebzade S. A validated method for analysis of proscillaridin in Drimia maritima (L.) stearn by high performance liquid chromatography. Tehran: Islamic Azad University, 2015