Advances in Profiling of Natural Products by Triple Detection Techniques Combined with Supercritical CO2 Mobile Phases and Sub 2-µm Stationary Phases

The analysis of natural products is challenging not only because of complex matrices and the presence of many compounds, but also because compounds with widely varying physical and chemical properties are usually present. To address these difficulties multiple detectors are often used. For example, mass spectrometry (MS) and ultraviolet (UV) detection are commonly combined. Evaporative light scattering detection (ELSD) may also be incorporated to address compounds without UV absorbance and which do not readily ionize. In this poster we investigate the analysis of Black Cohosh and a Glucosamine/Vitamin D dietary supplement using supercritical-fluid chromatography and triple detection techniques. We show that each detection technique is able to identify compounds undetected by the other techniques and may be necessary for complete characterization of natural products extracts and products.