Planta Med 2016; 82 - PA25
DOI: 10.1055/s-0036-1578640

Quantitative Determination Of Saccharin, Aspartame, Acesulfame, Sucralose, Neotame, Advantame, And Three Steviol Glycosides In Foods And Supplements By LC-UV-ELSD/MS

YH Wang 1, S Hu 1, 2, B Avula 1, M Wang 1, S Sagi 1, IA Khan 1, 3
  • 1National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University, MS 38677, USA
  • 2School of Pharmacy, Shanxi Medical University, Taiyuan, 030001, P.R. China
  • 3Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS 38677, USA

High-intensity sweeteners are commonly used in foods, beverages, drugs and dietary supplements as alternatives to sugar, to provide sweet taste, and for lowering total calories of these products [1]. In the United States, six high-intensity sweeteners including acesulfame potassium, saccharin, aspartame, sucralose, neotame and advantame and certain steviol glycosides obtained from leaves of Stevia rebaudiana (Bertoni) are permitted by USFDA. However, cyclamate and neohesperidine dihydrochalcone are not approved in the US but authorized in the European Union [2, 3].

With the aim of quality assurance and adulterant assessment of high-intensity sweeteners in foods and supplements, an UHPLC-UV-ELSD/MS method was developed for the quantitative determination of eleven high-intensity sweeteners. These analytes including cyclamate sodium, neohesperidin dihydrochalcon, and nine topical sweeteners in the title were baseline separated in a 13 min run. The method was validated for extraction solvent, accuracy, precision, limits of detection (LOD) and limits of quantification (LOQ). This method was applied to the analysis of 67 various products including yogurt, drinks, powders and liquid extracts of stevia.

Fig. 1: Chromatogram of Mixture of 11 Standards (ELS Detection)

Acknowledgements: This research is supported in part by “Science Based Authentication of Dietary Supplements” funded by the Food and Drug Administration grant number 1U01FD004246 – 05 and the United States Department of Agriculture, Agricultural Research Service, Specific Cooperative Agreement No. 58 – 6408 – 1-603 – 07.

References: [1] Zygler A., Wasik A., et al., (2012) Food Addit. Contam. A. 29: 1391 – 1401. [2] United States Food and Drug Administration, High-Intensity Sweeteners (www.fda.gov/Food/Ingredients PackagingLabeling/FoodAdditivesIngredients/ ucm397716.htm) [3] Shah R., Farris S., et al., (2015) Food Addit. Contam. A. 32: 141 – 151.