Planta Med 2016; 82 - PC48
DOI: 10.1055/s-0036-1578750

Isolation Of Flavonoids And 2D-DOSY NMR Of Flavonol Mixture From Mesembryanthemum Forsskaolii Hochst. Ex. Boiss

A Moawad 1, E Amin 1, 2, R Mohamed 1
  • 1Pharmacognosy Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514, Egypt
  • 2Pharmacognosy Department, Faculty of Pharmacy, Al Jouf University, Sakaka, KSA

During the course of isolation of flavonoids from Mesembryanthemum forsskaolii Hochst. Ex. Boiss (family Aizoaceae); apigenin (1), kaempferol-3-O-glucoside (2), isohamnetin-3-O-glucoside (3), apigenin-7-O-glucoside (4) and rutin (5) were isolated and identified. A mixture of isorhamnetin-3-O-rutinoside (6) and kampherol-3-O-rutinoside (7) was obtained. The analysis of a complex mixture can be simplified by the use of Diffusion-Ordered Spectroscopy (DOSY)[1]; 2D-DOSY experiment was performed for the mixture; however very poor separation of peaks was obtained maybe due to the close molecular weight of the mixture components and their structure similarity. The complete assignment of the mixture components was performed without separation by the intensive study of HSQC and HMBC spectra. All the isolated compounds are reported for the first time from this plant.

References: [1] Balayssac S. et al. (2009)J Pharm Biomed Anal 50:602 – 612.