Planta Med 2016; 82 - PC51
DOI: 10.1055/s-0036-1578753

Oleanane And Ursane Saponins From The Roots Of Glandularia X Hybrida

NM Mohamed 1, 2, MA Makboul 2, SF Farag 2, SA Ross 1, 3
  • 1National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University, Mississippi 38677, United States
  • 2Pharmacognosy Department, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
  • 3Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, University, Mississippi 38677, United States

Bioassay guided fractionation of the roots of Glandularia x hybrida (Groenl. & Ruempler) Nesom & Pruski (Verbenaceae) resulted in isolation of oleanane and ursane triterpene derivatives. Three new saponins along with five triterpene derivatives were isolated. The structures of the isolated metabolites were elucidated based on comprehensive 1-D and 2-D NMR spectroscopic data as well as HR-ESI-MS. The isolated compounds were identified as 3-O-β-D-xylopyranoside-hedragenin-28-β-D-glucopyranosyl-(1→6)-[β-D-glucopyranosyl-(1→2)]-β-D-glucopyransyl ester (1), 3-O-β-D-xylopyranoside-hedragenin-28-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl ester (2), 23-acetoxy-3-O-β-D-xylopyranoside-pomolic acid-28-β-D-glucopyranosyl ester (3), 3-O-β-D-xylopyranoside-hedragenin-28-oic acid (4), 3-O-β-D-xylopyranoside-pomolic acid-28-β-D-glucopyranosyl ester (5), hedragenin (6), 3α,24β-dihydroxy-olean-12-en-28-oic acid (7) and ursolic acid (8).

Fig. 1

R

1

β-D-gluc-(1→6)-[β-D-gluc-(1→2)]-β-D-gluc

2

β-D-gluc-(1→2)-β-D-gluc

4

H

Fig. 2

R

3

Acetyl

5

CH3

Acknowledgment: The project was supported by the Egyptian Government and National Center for Natural Product Research.