Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1597048
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Method development and validation for optimized separation of the major polyphenolics in propolis extracts using GC-MS method

Authors

  • K Siemionow

    1   Department of Pharmaceutical Analysis, Medical University of Bialystok, ul. Mickiewicza 2 d, 15 – 222 Bialystok, Poland

  • M Tomczyk

    2   Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Bialystok, ul. Mickiewicza 2a, 15 – 230 Bialystok, Poland

  • U Czyzewska

    1   Department of Pharmaceutical Analysis, Medical University of Bialystok, ul. Mickiewicza 2 d, 15 – 222 Bialystok, Poland

  • W Miltyk

    1   Department of Pharmaceutical Analysis, Medical University of Bialystok, ul. Mickiewicza 2 d, 15 – 222 Bialystok, Poland
Further Information

Publication History

Publication Date:
14 December 2016 (online)

Also available at
 

Propolis has been used since ancient times in folk medicine [1]. It is a popular medicine possessing a broad spectrum of biological activities such as antibacterial, antiviral, antifungal, antioxidant and anticancer agent [2,3]. This material is one of the richest sources of polyphenolics [4]. Due to pharmacological properties it is used in the commercial production of dietary supplements. In this study, we developed and validated a new GC-MS method for quantifying flavonoids: chrysin, galangin, pinocembrin and phenolic acids: caffeic, p-coumaric as well as ferulic. Quantitative analysis of phenolic compounds was performed on HP-5MS a capillary column (30 m x 0.25 mm i.d., 0.25 µm). The column temperature was initially held at 50 °C for 10 min, and then the temperature was raised to 310 °C at rate of 2 °C/min, followed by an isothermal period of 10 min. The mass spectrometer was operated in electron impact ionization/selective ion monitoring (EI/SIM) mode. A sensitive, rapid and accurate GC-MS method was applied to analyse the different sorts of commercially available propolis extract as well as to the samples of raw propolis extracts obtained from beekeepers. Total flavonoids ranged from 15.60 to 72.51 mg/g and total phenolic acid from 6.28 to 133.60 mg/g. In one sample, chrysin was found at 90.33 mg/g contents. The most abundant p-coumaric acid was detected with highest level 42.07 mg/g. The method was found to be relatively simple, specific, precise and accurate and may be used for the quality control of simultaneous determination of polyphenolics in propolis extracts but also in other similar plant materials. This method also represents a reliable and useful tool for comprehensive multi-component analysis of polyphenols in propolis extracts used in apitherapy.

Acknowledgements: The study was conducted with the use of equipment purchased by Medical University of Bialystok as part of the OP DEP 2007 – 2013, Priority Axis I.3, contract No. POPW.01.03.00 – 20 – 008/09 and financially supported by the Medical University of Bialystok, Poland (KNOW Project No.124/KNOW/15).

Keywords: Propolis, polyphenols, GC-MS.

References:

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[2] Toreti VC, Sato HH, Pastore GM, Park YK. Recent progress of propolis for its biological and chemical compositions and its botanical origin. Evid Based Complement Alternat Med 2013:697390.

[3] Sforcin JM, Bankova V. Propolis: is there a potential for the development of new drugs? J Ethnopharmacol 2011; 133: 253 – 260

[4] Czyzewska U, Kononczuk J, Teul J, Dragowski P, Pawlak-Morka R, Surazynski A, Miltyk W. Verification of chemical composition of commercially available propolis extracts by gas chromatography-mass spectrometry analysis. J Med Food 2015; 18: 584 – 591