Cannabinoid content in industrial hemp (Cannabis sativa L.) varieties grown in Slovenia

Hemp (Cannabis sativa L., subsp. sativa) contains cannabinoids that are of increasing scientific interest, the most known among them are cannabidiol (CBD) and (-)-trans-Δ9-tetrahydrocannabinol (Δ9-THC) with known therapeutic activities [1]. Fifteen varieties of common hemp from the ‘Common catalogue of varieties of agricultural plant species’ were analyzed: Fedora 17, KC Dora, Monoica, USO 3, Helena, Santhica 27, Tisza, Tiborszallasi, Antal, Carmagnola, Finola, Kompolti hibrid TC, Ferimon, Novosadska, Marina. Contents of cannabinoids Δ8-THC, Δ9-THCA, Δ9-THC, CBC, CBDA, CBD, CBDVA, CBDV, CBGA, CBG, CBL, CBN, THCV were determined by a validated HPLC method in bracts, remaining parts of inflorescences and whole inflorescences of each plant (n=3). Contents of cannabinoids were highest in bracts. Contents of total CBD+CBDA in whole inflorescences were highest in: Helena (2,4%), Tisza (1,9%), Antal (1,8%), Fedora 17 (1,6%) and Tiborszallasi (1,6%). The highest Δ9-THC+Δ9-THCA content was found in KC Dora (0,9%), but lower than 0,2% in other varieties. CBG+CBGA prevailed in Santhica 27 (3,1%), with no significant amounts of other cannabinoids. For varieties Fedora 17, USO 31, Tisza, Tiborszallasi, and Antal, CBD content in whole inflorescences was comparable to the declared values. Uniformity of Δ9-THC/CBD ratio was shown for the varieties Helena (1:31), Kompolti hibrid TC (1:33), Finola (1:29), Monoica (1:29), and USO 31 (1:33). Less uniform Δ9-THC/CBD ratio was observed in Carmagnola (1:1 to 1:32), KC Dora (1:1 to 1:33), Tiborszallasi (1:1 to 1:30), and Tisza (1:1 to 1:31).

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Artikel online veröffentlicht:
13. Dezember 2021

• References

• 1 Hillig K.W., Mahlberg P.G. A chemotaxonomic analysis of cannabinoid variation in Cannabis (Cannabaceae). Am. J. Bot. 91 2004; pp. 966-975 DOI: 10.1002/cncr.25812.