Planta Med 2021; 87(15): 1300
DOI: 10.1055/s-0041-1736942
Abstracts
8. Poster Contributions
8.9 Recent Advances in Medicinal Plant and Natural Product Research

Primula veris from Epirus, Greece: a rich source of flavonoids. Development and validation of an HPLC method for the analysis of the flowers

P I Chintiroglou
1   Laboratory of Pharmacognosy, School of Pharmacy, Aristotle University of Thessaloniki, University Campus, 54124, Thessaloniki, Greece
,
N Krigas
2   Hellenic Agricultural Organization – DEMETER, Institute of Breeding and Plant Genetic Resources, Thermi, 57001, Greece.
,
P Chatzopoulou
2   Hellenic Agricultural Organization – DEMETER, Institute of Breeding and Plant Genetic Resources, Thermi, 57001, Greece.
,
A Karioti
1   Laboratory of Pharmacognosy, School of Pharmacy, Aristotle University of Thessaloniki, University Campus, 54124, Thessaloniki, Greece
› Author AffiliationsThis study is funded by the Region of Epirus, Greece. The authors are grateful to Assistant Professor Maria Halabalaki, National and Kapodestrian University of Athens for recording the High-Resolution mass spectra.
› Further Information
 

An HPLC-PDA method was developed for the determination of the flavonoids, in the flowers of Primula veris from Epirus, Greece. The aim was to investigate the chemical content of the over harvested Primula veris populations and to develop a suitable analysis protocol. Primula flowers from Epirus were particularly rich in flavonol triglycosides, derivatives of quercetin, isorhamnetin and kaempferol (1-3). Chromatographic isolations of the hydromethanolic (70%) extract afforded a new naturally occurring flavonoid, isorhamnetin-3-Ο-β-glucopyranosyl-(1→2)-β-glucopyranosyl-(1→6)-β-glucopyranoside (3). Its structure elucidation was carried out by 1D and 2D NMR and mass spectrometry analyses. An HPLC-UV method was developed and validated according to ICH guidelines. Rutin was used as secondary standard and the correction factor for response was determined. The HPLC method was validated for linearity, LOD, LOQ precision and accuracy in three concentration levels. R.S.D. values ranged between 0.18% and 2.67%, for the intraday variation, within the acceptable limits. The inter-day variation ranged between 0.22% and 3.37% R.S.D. Accuracy was between 96.8 and 104.4 with RSD values ranging between 0.62 and 4.20. Finally, the recovery of the extraction was proved to be over 99.5% after four extraction cycles, while the precision of the extraction expressed as RSD values varied between 1.58 and 4.66, within the acceptable limits. The developed assay was fast and simple and permitted the quality control of the herbal drug. The studied samples were particularly rich in flavonoids and the mean total content of flavonols ranged from 4.46-6.67%mg, higher than the 3% which is reported by the EMA [1].

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Publication History

Article published online:
13 December 2021

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  • References

  • 1 EMA/HMPC/104095/2012 Committee on Herbal Medicinal Products (HMPC) Community herbal monograph on Primula veris L. and/or Primula elatior (L.) Hill, radix
    PubMed