Planta Med 2021; 87(15): 1304
DOI: 10.1055/s-0041-1736956
Abstracts
8. Poster Contributions
8.9 Recent Advances in Medicinal Plant and Natural Product Research

Cytotoxic effect on human melanoma cell lines and tyrosinase inhibition of Hottonia palustris

Podolak 2
J W Strawa
1   Department of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, Poland
,
A Galanty
2   Department of Pharmacognosy, Faculty of Pharmacy, Jagiellonian University Medical College, ul. Medyczna 9, 30-688 Cracow, Poland
,
K Jakimiuk
1   Department of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, Poland
,
K Grabowska
2   Department of Pharmacognosy, Faculty of Pharmacy, Jagiellonian University Medical College, ul. Medyczna 9, 30-688 Cracow, Poland
,
M Tomczyk
1   Department of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, Poland
› Author Affiliations
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Our previous study on Hottonia palustris L. (Primulaceae) [1] lead to isolation and identification of rare methoxyflavone derivatives, including 5-hydroxyflavone, 5-hydroxy-2′-methoxyflavone, 5-hydroxy-2′,6′-dimethoxyflavone, as well as dibenzoylmethane [2], and sakurasosaponin. Thus, in the present study the activity of the isolated compounds, together with crude extracts and fractions was investigated, with a focus on an in vitro melanoma model. HTB140, A375, WM793 human melanoma cells, combined with normal skin keratinocytes HaCaT were used to examine the efficacy towards cancer cells differing in metastatic potential, as well as to verify the selectivity of action. Cell viability test was performed with the MTT assay, after 24h and 48h of incubation [3]. Butanolic fraction and the isolated sakurasosaponin revealed the highest cytotoxicity, with IC50 7.15-16.63 and 2.04-4.5 µg/mL, respectively, and high selectivity. As melanoma growth is also associated with hyperexpression of tyrosinase, inhibitory activity towards this enzyme was investigated. Diethyl ether and ethyl acetate fractions revealed the highest activity, with IC50 59.88 and 75.14 µg/mL, respectively. Such comprehensive anti-melanoma approach provides a more precise answer to the impact of the tested compounds on the viability of cancer cells, also their selectivity action on normal cells what may lead to preselection of substances with both high efficacy and safety profile.



Publication History

Article published online:
13 December 2021

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