Planta Med 2021; 87(15): 1313
DOI: 10.1055/s-0041-1736984
Abstracts
8. Poster Contributions
8.9 Recent Advances in Medicinal Plant and Natural Product Research

Phytochemical analysis of Gnetum montanum stems’ ethanol extract and in vitro evaluation of tyrosinase and elastase inhibitory activity.

CT N Nguyen
1   Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, NKUA, Athens, Greece
2   Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh, Vietnam
,
L Antoniadi
1   Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, NKUA, Athens, Greece
,
H Tran
2   Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh, Vietnam
,
T N Huynh
2   Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh, Vietnam
,
H Stuppner
3   Department of Pharmacognosy, Institute of Pharmacy, University of Innsbruck, Austria
,
A Angelis
1   Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, NKUA, Athens, Greece
,
M Halabalaki
1   Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, NKUA, Athens, Greece
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Gnetum montanum Markgr.(Gnetaceae) is used in Vietnamese traditional medicine for the treatment of rheumatoid, arthritis and bronchitis [1] while recently has been reported as an effective plant in the treatment of gout in Vietnam [2]. Our study focused on isolation and identification of natural compounds from stems of G. montanum and evaluation of their inhibitory activity on tyrosinase and elastase enzymes.

The dry stems were initially extracted with ethanol 96% and subsequently the ethanol extract was partitioned with CHCl3, EtOAc and H2O and the fractions were evaluated in vitro for their capacity to inhibit tyrosinase and elastase enzymes. The EtOAc fraction presented high anti-tyrosinase and anti-elastase activity with IC50=0.011mg/ml and 0,072mg/ml, respectively and was chosen for further phytochemical analysis.

UPLC-HRMS/MS analysis of the above fraction resulted in the identification of over than 60 secondary metabolites, which mainly belong to stilbenoids, lignans, flavonoids, simple phenolics, and alkaloids. The combination of Centrifugal Partition Chromatography (with a series of 4 biphasic systems composed of n-hex-EtOAc-isoPrOH-H2O x/y/4/6) and prep- HPLC or prep-TLC led to the isolation of 13 natural compounds, of which three aroma acids (p-hydroxybenzoic acid, 3-(4-hydroxyphenyl)-propionic acid, vanillic acid), three flavonoids (hesperidine, vitexin, nobiletine) and seven stilbenoids (2b-hydroxyampelopsin F, isorhapotogenin, resveratrol, ε-viniferine, gnetol, gnemontanin G and gnetanin A). To the best of our knowledge, this is the first report of the trimeric stilbene gnetanin A. All isolated compounds were elucidated by using 1D and 2D NMR spectroscopy as well HRMS/MS spectrometry and then their inhibition activity against tyrosinase and ellastase was investigated



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Artikel online veröffentlicht:
13. Dezember 2021

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  • References

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