Planta Med 2021; 87(15): 1314
DOI: 10.1055/s-0041-1736987
Abstracts
8. Poster Contributions
8.9 Recent Advances in Medicinal Plant and Natural Product Research

In vitro anti-inflammatory potential and chemical profiling of Entada africana from the Republic of Benin

MurielleCodo Toafode
1   University of IRGIB Africa, Cotonou, Benin
4   University of Leipzig, Institute of Pharmacy, Germany
,
Verena Spiegler
2   University of Münster, Institute for Pharmaceutical Biology and Phytochemistry (IPBP), Germany
,
Cica Vissiennon
3   University of Leipzig, Institute for Medical Physics and Biophysics, Leipzig, Germany
6   REPHA GmbH Biologische Arzneimittel, Langenhagen, Germany
,
Virgile Ahyi
1   University of IRGIB Africa, Cotonou, Benin
,
Karin Fester
4   University of Leipzig, Institute of Pharmacy, Germany
5   University of Applied Sciences Zittau/Görlitz, Zittau, Germany
› Author Affiliations
DAAD (German Academic Exchange Service, Funding programme /- ID: 57299293).
› Further Information
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Entada africana Guill. et Perr, a plant of the Fabaceae family, has been reported to exhibit potential analgesic efficacy in the management of inflammatory-related conditions in traditional medicine in the Republic of Benin [1] [2]. However, there is still a lack of knowledge about the constituents of the plant's leaves. The objective of the present study was to assess the anti-inflammatory effect of a hydroalcoholic leaf extract of E. africana and its fractions and to identify its active compounds. Fractions were obtained by chromatography on Sephadex® LH-20 using a step gradient from ethanol, methanol/water, and methanol to acetone. Subsequently, fractions were screened for their phenolic profile using thin-layer chromatography and UHPLC-DAD. Then, in vitro anti-inflammatory assays were carried out with fractions FC3, FC4, FC7, FC8, and FC22 using a TNFα-stimulated human keratinocyte cell line (HaCaT) as a skin inflammation model. FC7 and FC22 significantly decreased the release of IL8 with IC50 91.74 µg/ml and IC50 63.47 µg/ml, respectively. In addition, fractions FC3 (IC50 54.13 µg/mL), FC4 (IC50 87.55 µg/ml), and FC22 (IC50 72.91 µg/ml) had a significant influence on IL6 release. Out of these fractions, ten phenolic compounds were isolated by MPLC on RP-18 and preparative HPLC. Structures were elucidated by HRESI-MS and NMR. The results obtained, support the anti-inflammatory effect of E. africana and its application in traditional Beninese medicine. These compounds will then be analyzed for their in vitro activity to identify compounds responsible for the pharmacological effect.



Publication History

Article published online:
13 December 2021

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