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Planta Med 2016; 82(08): 747-751
DOI: 10.1055/s-0042-104202
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Detection of Ganoderic Acid A in Ganoderma lingzhi by an Indirect Competitive Enzyme-Linked Immunosorbent Assay

Seiichi Sakamoto   *
1   Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, Fukuoka, Japan
,
Toshitaka Kohno   *
1   Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, Fukuoka, Japan
,
Kuniyoshi Shimizu
2   Department of Agro-Environmental Sciences, Graduate School of Agriculture, Kyushu University, Higashi-ku, Fukuoka, Japan
,
Hiroyuki Tanaka
1   Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, Fukuoka, Japan
,
Satoshi Morimoto
1   Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, Fukuoka, Japan
› Author Affiliations
Further Information

Publication History

received 08 November 2015
revised 16 February 2016

accepted 24 February 2016

Publication Date:
19 April 2016 (online)

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Abstract

Ganoderma is a genus of medicinal mushroom traditionally used for treating various diseases. Ganoderic acid A is one of the major bioactive Ganoderma triterpenoids isolated from Ganoderma species. Herein, we produced a highly specific monoclonal antibody against ganoderic acid A (MAb 12 A) and developed an indirect competitive ELISA for the highly sensitive detection of ganoderic acid A in Ganoderma lingzhi, with a limit of detection of 6.10 ng/mL. Several validation analyses support the accuracy and reliability of the developed indirect competitive ELISA for use in the quality control of Ganoderma based on ganoderic acid A content. Furthermore, quantitative analysis of ganoderic acid A in G. lingzhi revealed that the pileus exhibits the highest ganoderic acid A content compared with the stipe and spore of the fruiting body; the best extraction efficiency was found when 50 % ethanol was used, which suggests the use of a strong liquor to completely harness the potential of Ganoderma triterpenoids in daily life.

Key words

Ganoderma lingzhi - Ganodermataceae - enzyme-linked immunosorbent assay - ganoderic acid A (GAA) - monoclonal antibody - quality control

* These authors contributed equally to this work.


Supporting Information

 

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