New Sulphated Flavonoids from Tamarix africana and Biological Activities of Its Polar Extract

Manel Karker; Nunziatina De Tommasi; Abderrazak Smaoui; Chedly Abdelly; Riadh Ksouri; Alessandra Braca

doi:10.1055/s-0042-111520

Planta Medica, Inhaltsverzeichnis

Planta Med 2016; 82(15): 1374-1380
DOI: 10.1055/s-0042-111520

Natural Product Chemistry and Analytical Studies

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

New Sulphated Flavonoids from Tamarix africana and Biological Activities of Its Polar Extract

Authors

Manel Karker

¹Laboratory of Aromatic and Medicinal Plants, Biotechnology Center at the Technopole of Borj-Cédria (CBBC), Hammam-Lif, Tunisia
Nunziatina De Tommasi

²Dipartimento di Farmacia, Università di Salerno, Fisciano (SA), Italy
Abderrazak Smaoui

³Laboratory of Extremophile Plants, Biotechnology Center at the Technopole of Borj-Cedria (CBBC), Hammam-Lif, Tunisia
Chedly Abdelly

³Laboratory of Extremophile Plants, Biotechnology Center at the Technopole of Borj-Cedria (CBBC), Hammam-Lif, Tunisia
Riadh Ksouri

¹Laboratory of Aromatic and Medicinal Plants, Biotechnology Center at the Technopole of Borj-Cédria (CBBC), Hammam-Lif, Tunisia
Alessandra Braca

⁴Dipartimento di Farmacia, Università di Pisa, Pisa, Italy

⁵Centro Interdipartimentale di Ricerca "Nutraceutica e Alimentazione per la Salute”, Università di Pisa, Pisa, Italy

Abstract

The phytochemical investigation of Tamarix africana Poir. (Tamaricaceae) shoot polar extract afforded three new sulphated flavonoids, (2S,4R)-5,7,4′-trihydroxyflavan-4-ol 5,7-disulphate (1), (2S)-5,7,4′-trihydroxyflavan 7-O-sulphate (2), and (2S)-naringenin 4′-O-sulphate (3), together with ten known compounds. Their structures were determined by spectroscopic methods including 1D and 2D NMR analysis and HRMS. Biological activities of the polar extract of T. africana shoots related to its phenolic content were also investigated. A high total phenolic content (151.1 mg GAE/g) was found in the methanol shoot extract, which exhibits strong antioxidant activities using the oxygen radical absorbance capacity method and a skin cell-based assay. Moreover, the shoot extract showed significant anti-inflammatory activity, reducing nitric oxide release by 53.5 % at 160 µg/mL in lipopolysaccharide-stimulated RAW 264.7 macrophages. Finally, T. africana shoot extract inhibited the growth of A-549 lung carcinoma cells, with an IC₅₀ value of 34 µg/mL.

Key words

Tamarix africana - Tamaricaceae - sulphated flavonoids - antioxidant activity - cytotoxicity - anti-inflammatory activity

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Referenzen

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