Planta Med 2022; 88(15): 1486-1487
DOI: 10.1055/s-0042-1759125
Poster Session I

Anti-inflammatory potential of phenolic compounds isolated from Entada africana Guill. & Perr. used in the Republic of Benin

Authors

  • NM Codo Toafode∗

    1   Inter-Regional University of Industrial Engineering Biotechnologies and Applied Sciences, IRGIB Africa University, C/493, 07 BP 231, Cotonou, Benin
    2   Institute for Medical Physics and Biophysics, Leipzig University, Härtelstraße 16 – 18, 04107, Leipzig, Germany

  • P Marquardt

    3   Faculty of Natural and Environmental Sciences, Zittau/Görlitz University of Applied Sciences, Theodor-Körner-Allee 16, 02763, Zittau, Germany

  • V Ahyi

    1   Inter-Regional University of Industrial Engineering Biotechnologies and Applied Sciences, IRGIB Africa University, C/493, 07 BP 231, Cotonou, Benin

  • K Fester

    3   Faculty of Natural and Environmental Sciences, Zittau/Görlitz University of Applied Sciences, Theodor-Körner-Allee 16, 02763, Zittau, Germany

  • V Spiegler

    4   Institute for Pharmaceutical Biology and Phytochemistry, University of Münster, Corrensstraße 48, 48149, Münster, Germany

  • C Vissiennon

    2   Institute for Medical Physics and Biophysics, Leipzig University, Härtelstraße 16 – 18, 04107, Leipzig, Germany
    5   Repha GmbH Biologische Arzneimittel, Alt-Godshorn 87, 30855, Langenhagen, Germany
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Entada africana is a West African tree with numerous ethnomedicinal uses. The bark and root of E. africana have been investigated for their phytochemical compositions. However, knowledge on phenolic composition of the leaves, which are predominantly used in the Republic of Benin for the treatment of wounds, fractures and sprains, and their bioactivity is still scarce. This study aims at elucidating phenolic compounds of a hydroalcoholic leaf extract of E. africana and assessing their bioactivity. Structural elucidation was performed using HRESI-MS and NMR methods. Bioactivity of E. africana crude extract, fractions thereof, and isolated compounds was evaluated using TNF-α stimulated human keratinocytes (HaCaT) as an in vitro model of skin inflammation. As a result, 11 phenolic compounds were identified in the hydroalcoholic leaf extract of E. africana, which moderately inhibited IL-8 release to 48.1 ± 3.5% with an IC50 = 59.2 µg/mL. Among the 11 phenolic compounds, 3′,4′,7-trihydroxyflavone showed a significant inhibitory effect on IL-6 interleukin with a maximum inhibition of 74.4 ± 2% and IC50 = 17.8 µg/mL and moderately reduced IL-8 interleukin secretion to 40.2 ± 5.1% with IC50 = 126.2 µg/mL. This study provides for the first time an overview of the phenolic compounds present in the hydroethanolic leaf extract of E. africana and their anti-inflammatory potential, which support its traditional medicinal use in the treatment of wounds, fractures and sprains.

The authors declare that there is no conflict of interest.



Publication History

Article published online:
12 December 2022

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