An UHPLC-MS based metabolomic approach to explore effects of bacterial endophytes on Alkanna tinctoria (L.) Tausch cell suspension metabolome

The plant endo-microbiome might influence the production of secondary metabolites (SMs). In this work, the interaction between Alkanna tinctoria (L.) Tausch cell suspension and eight of its bacteria endophytes was investigated through an Ultra-High-Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS) untargeted metabolomics approach ([Fig. 1]). Principal component analysis and hierarchical clustering heat map were applied to the data analysis to visualize the potential modification of the metabolome. The bacteria endophyte-induced metabolites were then putative classified and identified using Molecular Networking. UHPLC-MS-based hierarchical clustering analysis, principal component analysis together with MS/MS molecular networking highlighted significant modifications in SMs production caused by bacteria endophytes.

Fig. 1 Influence of bacterial endophytes on Alkanna tinctoria.

This work has been financed by the EU H2020-ITN-MICROMETABOLITE project (Grant N°721 635).

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Artikel online veröffentlicht:
12. Dezember 2022