Planta Med 2022; 88(15): 1565
DOI: 10.1055/s-0042-1759327
Poster Session II

The potential of biotechnological studies on chia (Salvia hispanica L.) in vitro cultures

S Motyka∗
1   Chair and Department of Pharmaceutical Botany, Faculty of Pharmacy, Collegium Medicum, Jagiellonian University, 9 Medyczna Street, 30 – 668, Kraków, Poland
2   Doctoral School of Medical and Health Sciences, Medical College, Jagiellonian University, Łazarza 16, Kraków, Poland
,
A Szopa
1   Chair and Department of Pharmaceutical Botany, Faculty of Pharmacy, Collegium Medicum, Jagiellonian University, 9 Medyczna Street, 30 – 668, Kraków, Poland
,
H Ekiert
1   Chair and Department of Pharmaceutical Botany, Faculty of Pharmacy, Collegium Medicum, Jagiellonian University, 9 Medyczna Street, 30 – 668, Kraków, Poland
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Salvia hispanica L. (Lamiaceae), is a species providing chia seeds, which, thanks to their rich chemical composition and valuable biological activities, are classified as so-called “healthy food” [1], [2].

The biotechnological experiments involved agitated and Plantform-bioteactor grown S. hispanica microshoot cultures maintained in Murashige-Skoog medium [3] with 2 mg/L6-benzyladenine. The stimulatory effect of culture media supplementation with precursor compound – L-phenylalanine (Phe), and the effect of elicitation with methyl jasmonate (MeJa), on the production of phenolic compounds was proved.

Eight compounds: quercetin, chlorogenic acid, cinnamic acid, caffeic acid, neochlorogenic acid, protocatechuic acid, syringic acid and rosmarinic acid (quantitatively dominant in all samples) were determined with an HPLC-DAD method [4] in the extracts from experimental cultures.

The study of the dynamic of phenolic compounds accumulation showed, that the total content of estimated compounds was dependent on the duration of culture growth period and ranged from 107.14 to 748.61 mg/100 g s. m.

The highest content of rosmarinic acid (4050.38 mg/100 g s. m.; 60 times higher than in the control), was found in the extracts of agitated cultures supplemented with 1 mmol/L Phe on 7th day of 13 days growth period.

Studies on Plantform grown microshoots also proved high production of rosmarinic acid (3261.08 mg/100 g DW) which was obtained after media supplementation with 3 mmol/l Phe on the 17th day of 25 days growth period.

S. hispanica in vitro cultures can be proposed as a source of phenolic compounds, especially rosmarinic acid, a compound with valuable antioxidant properties. I declare no conflict of interest.



Publication History

Article published online:
12 December 2022

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