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Planta Med 2018; 84(02): 117-122
DOI: 10.1055/s-0043-116853
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

ISSR-Derived Species-Specific SCAR Marker for Rapid and Accurate Authentication of Ocimum tenuiflorum L.

Amit Kumar
1   Department of Plant Biology and Systematics, CSIR – Central Institute of Medicinal and Aromatic Plants, Research Centre, Bengaluru, India
,
Vereena Rodrigues
1   Department of Plant Biology and Systematics, CSIR – Central Institute of Medicinal and Aromatic Plants, Research Centre, Bengaluru, India
,
Priyanka Mishra
1   Department of Plant Biology and Systematics, CSIR – Central Institute of Medicinal and Aromatic Plants, Research Centre, Bengaluru, India
,
Kuppusamy Baskaran
1   Department of Plant Biology and Systematics, CSIR – Central Institute of Medicinal and Aromatic Plants, Research Centre, Bengaluru, India
,
Ashutosh K. Shukla
2   Biotechnology Division, CSIR – Central Institute of Medicinal and Aromatic Plants, Lucknow, India
,
Ajit K. Shasany
2   Biotechnology Division, CSIR – Central Institute of Medicinal and Aromatic Plants, Lucknow, India
,
Velusamy Sundaresan
1   Department of Plant Biology and Systematics, CSIR – Central Institute of Medicinal and Aromatic Plants, Research Centre, Bengaluru, India
› Author Affiliations
Further Information

Publication History

received 31 March 2017
revised 03 July 2017

accepted 11 July 2017

Publication Date:
24 July 2017 (online)

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Abstract

Ocimum tenuiflorum has been widely used in traditional medicine and has high medicinal value. High volume trade of this potential medicinal plant species led to unscrupulous adulteration of both crude drugs as well as formulations. Morphology-based authentication is difficult in cases of incomplete or damaged samples and in dried herbal materials. In such cases, PCR-based molecular methods may aid in accurate identification. The present study aimed at developing species-specific DNA marker(s) for the authentication of O. tenuiflorum. A species-specific amplicon (279 bp) generated through an inter-simple sequence repeat marker (UBC 835) in all individuals of O. tenuiflorum was cloned, sequenced, and a primer pair was developed (designated as CIM-OT-835F/CIM-OT-835R). The newly developed sequence characterized amplified region marker was validated through PCR amplification in all available seven species of Ocimum, and its specificity for O. tenuiflorum was confirmed with the consistent generation of an amplicon of 177 bp. The developed marker can be used for accurate and rapid identification of the species for certification purposes and will be useful in quality control of medicinal preparations containing this important medicinal species.

Key words

adulteration - ISSR - Lamiaceae - Ocimum species - SCAR marker - authentication
 

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