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Planta Med 2018; 84(05): 296-303
DOI: 10.1055/s-0043-120271
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Farrerol Modulates Aorta Gene Expression Profile in Spontaneously Hypertensive Rats

Xiaojiang Qin
1   School of Public Health, Shanxi Medical University, Taiyuan, Shanxi, China
,
Xiaomin Hou
2   Department of Pharmacology, Shanxi Medical University, Taiyuan, Shanxi, China
,
Kun Zhang
3   School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, Shanxi, China
,
Qingshan Li
3   School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, Shanxi, China
4   Shanxi University of Chinese medicine, Taiyuan, Shanxi, China
› Institutsangaben
Weitere Informationen

Publikationsverlauf

received 23. Juni 2017
revised 12. September 2017

accepted 18. September 2017

Publikationsdatum:
06. Oktober 2017 (online)

    Lizenzen und Reprints

Abstract

Farrerol, a typical natural flavanone and major active component in Rhododendron dauricum var. ciliatum, has been shown to possess vasoactive ability in vitro. The aim of this study was to investigate its effect on aorta gene expression in spontaneously hypertensive rats. Twelve-week-old male normotensive Wistar Kyoto rats and spontaneously hypertensive rats were treated with orally administered farrerol (50 mg/kg body weight) for 8 wk before they were sacrificed. We found that aorta samples showed 444 upregulated genes in control spontaneously hypertensive rats compared with the control Wistar Kyoto rats. Administration of farrerol in spontaneously hypertensive rats increased the expression of 2329 genes in the aorta compared with the control spontaneously hypertensive rats. Gene expression profiles performed on the aorta revealed that farrerol induced changes in vascular smooth muscle contraction, mitogen-activated protein kinase signaling pathway, regulation of actin cytoskeleton, vascular endothelial growth factor signaling pathway, calcium signaling pathway, and renin angiotensin system. Furthermore, 10 genes involved in the pathway of vascular smooth muscle contraction were verified using real-time polymerase chain reaction technique, and several novel potential target genes for the farrerol treatment of hypertension were identified. The findings of this study lend support to the potential use of farrerol as a novel therapeutic and antihypertensive candidate drug to prevent the development of hypertension.

Key words

flavanone - Ericaceae - farrerol - Rhododendron dauricum - gene expression profile - aorta - spontaneously hypertensive rat

Supporting Information

    Assessment of the degree of saturation in DEGs sequencing, distribution of total and distinct tags over different tag-abundance categories, and histogram presentation of GO classification are available as Supporting Information.

  • Supporting Information
 

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