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Planta Med 2018; 84(08): 536-543
DOI: 10.1055/s-0043-123655
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

A Simple and Rapid UPLC-PDA Method for Quality Control of Nardostachys jatamansi

Weize Zhang
1   Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Chemistry and Analysis of Traditional Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China
,
Guo Nan
1   Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Chemistry and Analysis of Traditional Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China
,
Hong-Hua Wu
1   Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Chemistry and Analysis of Traditional Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China
,
Miaomiao Jiang
1   Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Chemistry and Analysis of Traditional Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China
,
Tian-Xiang Li
2   Chinese Medicine Research Center, Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China
,
Meng Wang
1   Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Chemistry and Analysis of Traditional Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China
,
Xiu-Mei Gao
1   Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Chemistry and Analysis of Traditional Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China
,
Yan Zhu
1   Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Chemistry and Analysis of Traditional Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China
,
Yun Seon Song
3   College of Pharmacy, Sookmyung Womenʼs University, Seoul, Korea
,
Jiaming Wang
4   Key Laboratory of Industrial Fermentation Microbiology Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, P. R. China
,
Yan-Tong Xu
1   Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Chemistry and Analysis of Traditional Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China
› Author Affiliations
Further Information

Publication History

received 12 May 2017
revised 09 November 2017

accepted 17 November 2017

Publication Date:
04 December 2017 (online)

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Abstract

Nardostachys jatamansi is a well-documented herbal agent used to treat digestive and neuropsychiatric disorders in oriental medicinal systems. However, few simple, rapid, and comprehensive methods were reported for quality assessment and control of N. jatamansi. Herein, a UPLC with photodiode array detection method was developed for both fingerprint investigation of N. jatamansi and simultaneous quantitative analysis of the six serotonin transporter modulatory constituents in N. jatamansi. For chromatographic fingerprinting, 24 common peaks were selected as characteristic peaks to assess the consistency of N. jatamansi samples from different retail sources. Six of the common peaks (5, 7, 12, and 16 – 18) were identified as desoxo-narchinol A, buddleoside, isonardosinone, nardosinone, kanshone H, and (−)-aristolone, respectively, by phytochemical investigation. Five of the six compounds significantly either enhanced or inhibited serotonin transporter activity, while (−)-aristolone (18) didnʼt show any serotonin transporter activity. In quantitative analysis, the six compounds showed good linearity (r > 0.999) within test ranges. The precision, expressed as relative standard deviation, was in the range of 0.25 – 2.77%, and the recovery of the method was in the range of 92 – 105%. The UPLC-photodiode array detection-based fingerprint analysis and quantitative methods reported here could be used for routine quality control of N. jatamansi.

Key words

Nardostachys jatamansi - Caprifoliaceae - UPLC-PDA - serotonin transporter - quality control

Supporting Information

    Experimental procedures for the isolation and identification of reference compounds, optimization of the extraction condition, and detailed results of similarity evaluation (Table 5S), UPLC-TOFMS analysis, method validation (Table 6S: calibration curves, detection limits, quantification limits; Table 7S: intra- and inter-day variability, repeatability, and recovery investigations), and data of quantitative analysis (Table 8S) are available as Supporting Information.

  • Supporting Information
 

  • References

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