Planta Med 2018; 84(11): 779-785
DOI: 10.1055/s-0044-100797
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Anti-Angiogenic Activity of Rotenoids from the Stems of Derris trifoliata

Yanisa Mittraphab
1   Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
,
Nattaya Ngamrojanavanich
1   Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
,
Kuniyoshi Shimizu
2   Department of Agro-Environmental Science, Faculty of Agriculture, Kyushu University, Fukuoka, Japan
,
Kiminori Matsubara
3   Department of Human Life Science Education, Graduate School of Education, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan
,
Khanitha Pudhom
1   Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
› Author Affiliations
Further Information

Publication History

received 03 November 2017
revised 27 December 2017

accepted 05 January 2018

Publication Date:
18 January 2018 (online)


Abstract

The plants in the genus Derris have proven to be a rich source of rotenoids, of which cytotoxic effect against cancer cells seem to be pronounced. However, their effect on angiogenesis playing a crucial role in both cancer growth and metastasis has been seldom investigated. This study aimed at investigating the effect of the eight rotenoids (18) isolated from Derris trifoliata stems on three cancer cells and angiogenesis. Among them, 12a-hydroxyrotenone (2) exhibited potent inhibition on both cell growth and migration of HCT116 colon cancer cells. Further, anti-angiogenic assay in an ex vivo model was carried out to determine the effect of the isolated rotenoids on angiogenesis. Results revealed that 12a-hydroxyrotenone (2) displayed the most potent suppression of microvessel sprouting. The in vitro assay on human umbilical vein endothelial cells was performed to determine whether compound 2 elicits anti-angiogenic effect and its effect was found to occur via suppression of endothelial cells proliferation and tube formation, but not endothelial cells migration. This study provides the first evidence that compound 2 could potently inhibit HCT116 cancer migration and anti-angiogenic activity, demonstrating that 2 might be a potential agent or a lead compound for cancer therapy.

Supporting Information

 
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