Georg Thieme Verlag Stuttgart · New York
Differentiation of Medicinal Codonopsis Species from Adulterants by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism
September 30, 1998
April 6, 1999
31 December 1999 (online)
DNA sequence analysis of rDNA internal transcribed spacer (ITS) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were exploited for their applications in differentiating medicinal species Codonopsis pilosula, C. tangshen, C. modesta, and C. nervosa var. macrantha, from two related adulterants Campanumoea javania and Platycodon grandiflorus. The data demonstrated that the rDNA ITSI and ITSII sequences of the four Codonopsis are highly homologous but not identical, and are significantly different from those of the two adulterants. The sequence difference allows effective and reliable differentiation of Codonopsis from the adulterants by PCR-RFLP.
Chinese medicine - Campanulaceae - Campanumoea javania - Codonopsis - C. modesta - C. pilosula - C. tangshen - Platycodon grandiflorus - Dangshen - Internal transcribed spacer (ITS) - PCR - rDNA - RFLP