Planta Med 2001; 67(6): 561-564
DOI: 10.1055/s-2001-16493

© Georg Thieme Verlag Stuttgart · New York

Antibacterial Activity of Hydrastis canadensis Extract and its Major Isolated Alkaloids

F. Scazzocchio1 , M.F. Cometa2,*, L. Tomassini3 , M. Palmery2
  • 1 Istituto di Microbiologia, Università ”La Sapienza”, Roma, Italia
  • 2 Dipartimento di Farmacologia delle Sostanze Naturali e Fisiologia Generale, Università ”La Sapienza”, Roma, Italia
  • 3 Dipartimento di Biologia Vegetale, Università ”La Sapienza”, Roma, Italia
Further Information

Publication History

October 6, 2000

January 21, 2001

Publication Date:
17 August 2001 (online)


The antibacterial activity of extract and isolated major alkaloids (berberine, β-hydrastine, canadine and canadaline) of Hydrastis canadensis L. (Ranunculaceae) was evaluated against 6 strains of microorganism: Staphylococcus aureus (ATCC 25 993 and ATCC 6538P), Streptococcus sanguis (ATCC 10 556), Escherichia coli (ATCC 25 922), Pseudomonas aeruginosa (ATCC 27 853). Bactericidal activity was evaluated by contact test by measuring the ”killing time” on a low density bacterial inoculum, and bacteriostatic activity in liquid medium by M.I.C. values. The results provide a rational basis for the traditional antibacterial use of Hydrastis canadensis.


  • 1 Lampe K F. Berberine. In: Adverse Effects of Herbal Drugs Vol 1. New York; Springer Verlag 1992: 97-104
  • 2 Chang H M, But P PH. In: Pharmacology and applications of Chinese materia medica. Vol II. Singapore; World Scientific 1986: 1029-40
  • 3 Amin A H, Subbaiah T V, Abbasi K M. Berberine sulfate: antimicrobial activity, bioassay, and mode of action. Can. J.  Microbiol,. 1969;  15 1067-76
  • 4 Hahn F E, Ciak J. Berberine. In: Corcoran, JW, Han, FE, (eds) Antibiotics; Vol 3. Mechanism of action of antimicrobial and antitumor agents. New York; Springer Verlag 1975: 577-84
  • 5 Pitea M, Margineanu C. Correlations between chemical structure and antibacterial activity of berberine.  Clujul Med,. 1972;  45 465-9
  • 6 Evans W C. Hydrastis In: Trease and Evans’ Pharmacognosy, 13th edn, Alkaloids. London; Baillièr Tyndall 1989: 592
  • 7 Reynolds J EF (ed). Hydrastis. In: Martindale, The Extra Pharmacopoeia. 31st edn. London; Royal Pharmaceutical Society 1996: 1714
  • 8 Messana I, La Bua R, Galeffi C. The alkaloids of Hydrastis canadensis L. (Ranunculaceae). Two new alkaloids: Hydrastidine and isohydrastidine Gazz. Chim.  Ital.. 1980;  110 539-43
  • 9 Galeffi C, Cometa M F, Tomassini L, Nicoletti M. Canadinic acid: An alkaloid from Hydrastis canadensis .  Planta Medica. 1997;  63 194
  • 10 Leone M G, Cometa M F, Palmery M, Saso L. HPLC Determination of the major alkaloids extracted from Hydrastis canadensis L.  Phytotherapy Research. 1996;  10 S45-6
  • 11 Palmery M, Cometa M F, Leone M G. Further studies of the adrenolytic activity of the major alkaloids from Hydrastis canadensis L. on isolated rabbit aorta.  Phytotherapy Research. 1996;  10 S47-9
  • 12 Palmery M, Cometa M F, Abdel-Haq H. Antiserotoninergic activity of the major alkaloids from Hydrastis canadensis L. on isolated rabbit aorta.  Pharmacological Research. 1997;  S35 28
  • 13 Bolle P, Cometa M F, Palmery M, Tucci P. Response of rabbit detrusor muscle to total extract and major alkaloids of Hydrastis canadensis .  Phytotherapy Research. 1998;  12 S86-8
  • 14 Cometa M F, Abdel-Haq H, Palmery M. Spasmolytic activities of Hydrastis canadensis L. on rat uterus and guinea-pig trachea.  Phytotherapy Research. 1998;  12 S83-5
  • 15 Palmery M, Abdel-Haq H, Cometa M F. Pharmacological studies of Hydrastis canadensis L. spasmolytic activity on guinea-pig trachea.  Pharmacological Research. 1999;  S39 37
  • 16 Reybrouck G. Evaluation of the antibacterial and antifungal activity of disinfectants. In: Principles and Practice of disinfection preservation and sterilisation. 2nd. ed. Blackwell Scientific Publication Oxford 1992: 114-33
  • 17 Seymour S. Block-Peroxigen compounds. In: Disinfection, Sterilization and Preservation. 4th ed. Lea & Febiger Philadelphia 1991: 167-81

Dr . M. F. Cometa

Dipartimento di Farmacologia delle Sostanze Naturali e Fisiologia Generale

Università di Roma ”La Sapienza”

P.le A. Moro, 5

00 185- Rome



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