Effect of Caffeic Acid on Apical Transporters’ Dysfunction of Renal Proximal Tubule Cells under Oxidative Stress in vitro

Abstract

The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10^-4 M H₂O₂ did not affect cell viability regardless of incubation time. However, it decreased apical transporters’ activity such as Na⁺/glucose cotransporter, Na⁺/Pi cotransporter, and Na⁺/H⁺ antiporter in the proximal tubule cells. CA (>10^-6 M) prevented H₂O₂-induced inhibition of apical transporters. Thus, we investigated its action mechanism. CA also prevented H₂O₂-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca²⁺ uptake. In conclusion, CA, in part, prevented H₂O₂-induced inhibition of apical transporter activity via decrease of AA release and Ca²⁺ uptake in primary cultured renal proximal tubule cells.

Abbreviations

AA:arachidonic acid

CA:caffeic acid

PTCs:proximal tubule cells

LPO:lipid peroxide

α-MG:α-methyl-D-glucopyranoside

Key words

Caffeic acid - apical transporter - kidney - H₂O₂ - arachidonic acid - Ca²⁺

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