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DOI: 10.1055/s-2002-34398
© Georg Thieme Verlag Stuttgart · New York
The Inhibitory Effects of Jujuboside A on Rat Hippocampus in Vivo and in Vitro
Publication History
Received: November 30, 2001
Accepted: March 23, 2002
Publication Date:
30 September 2002 (online)

Abstract
Jujuboside A (JuA) is a glycoside extracted from the seed of Ziziphus jujuba Mill var. spinosa (Bunge) Hu ex H F Chou, a Chinese herbal medicine, which has long been known as a sedative-hypnotic drug. The present study used the method of intracerebroventricular (i. c. v.) JuA in vivo to detect the effect of JuA on paired-pulse responses of dentate gyrus granule cells in urethane-anaesthetized rats, and the method of hippocampal slice bathing in JuA in vitro to detect the effects of JuA on the responses of CA1 pyramidal cells. The results showed that JuA significantly decreased the slopes of excitatory postsynaptic potential (EPSP) and the amplitudes of population spike (PS) in the first responses of granule cells and significantly decreased EPSP and PS in the responses of CA1 pyramidal cells. The good quantitative correspondence between in vitro and in vivo results indicates that studies using hippocampal formation allow research on the inhibitory effects of JuA in the rat central nervous system (CNS). In conclusion, our study accessed the inhibitory effects of JuA on hippocampal formation in vivo and in vitro, which contributed to the further research on the mechanism of its effects on CNS.
Abbreviations
ACSF:artificial cerebrospinal fluid
CaM:calmodulin
CNS:central nervous system
DG:dentate gyrus
EPSP:excitatory postsynaptic potential
EPSP1:the slope of the EPSP of the first pulse
EPSP2:the slope of the EPSP of the second pulse
GABA:γ-amino-butyric acid
i.c.v.:intracerebroventricular
JuA:jujuboside A
NGF:nerve growth factor
NMDA:N-methyl-D-aspartic acid
PP:perforant pathway
PS:population spike
PS1:the amplitude of PS of the first pulse
PS2:the amplitude of PS of the second pulse
PSL1:the latency of the PS1
PSL2:the latency of the PS2
Ziziphus:Ziziphus jujuba Mill var. spinosa (Bunge) Hu ex H F Chou
Key words
Jujuboside A - Ziziphus jujuba - Rhamnaceae - hippocampal formation - inhibitory effects
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Prof. Xiaoxiang Zheng
Department of Biomedical Engineering
Zhejiang University
Hangzhou
P.R.China 310027
Email: zxx@mail.hz.zj.cn
Fax: +86-571-87951676