Planta Med 2003; 69(10): 953-956
DOI: 10.1055/s-2003-45108
© Georg Thieme Verlag Stuttgart · New York

In Vitro Antiviral Activity of Prodelphinidin B-2 3,3′-Di-O-gallate from Myrica rubra

Hua-Yew Cheng1 , Ta-Chen Lin2 , Kanji Ishimaru3 , Chien-Min Yang1 , Kuo-Chih Wang4 , Chun-Ching Lin1 , 4
  • 1Graduate Institute of Pharmaceutical Sciences, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, R.O.C.
  • 2Department of Pharmacy, Tajen Institute of Technology, Ping-Tung, Taiwan, R.O.C.
  • 3Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, Saga, Japan
  • 4Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, R.O.C.
Further Information

Publication History

Received: March 4, 2003

Accepted: June 17, 2003

Publication Date:
02 December 2003 (online)


In this study, the in vitro antiviral properties of prodelphinidin B-2 3,3′-di-O-gallate (PB233′OG) isolated from the bark of Myrica rubra (Myricaceae) was investigated. Results showed that PB233′OG exhibited anti-herpes simplex virus type 2 (HSV-2) activity with IC50 values of 5.3 ± 0.1 and 0.4 ± 0.04 μM for XTT and plaque reduction assays, respectively. The IC50 value increased with increasing MOI (multiplicity of infection). PB233′OG did not show a cellular cytotoxic effect at concentrations that possessed antiviral activity. Mechanistic studies demonstrated that PB233′OG inhibited HSV-2 attachment to the Vero cell, interfered with the penetration of HSV-2 into the Vero cell, affected the late stage(s) of the HSV-2 infection cycle, and also reduced the viral infectivity at high concentrations. It is concluded that PB233′OG exhibits various modes of action in its anti-HSV-2 effects.


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Prof Chun-Ching Lin

Graduate Institute of Pharmaceutical Sciences

College of Pharmacy

Kaohsiung Medical University

807 Kaohsiung


Republic of China

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