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DOI: 10.1055/s-2004-835843
© Georg Thieme Verlag KG Stuttgart · New York
Inhibitory Effects of Centella asiatica Water Extract and Asiaticoside on Inducible Nitric Oxide Synthase During Gastric Ulcer Healing in Rats
Publikationsverlauf
Received: March 12, 2004
Accepted: June 27, 2004
Publikationsdatum:
10. Januar 2005 (online)
Abstract
In this study, the effects of Centella asiatica water extract (CE) and its active constituent, asiaticoside (AC), on the expression and activity of inducible nitric oxide synthase (iNOS) during gastric ulcer healing in rats were investigated. CE was prepared from Centella asiatica dry plant and the concentration of AC in CE was quantitatively determined with the use of high performance liquid chromatography analysis. Different concentrations of CE (0.10 g/kg and 0.25 g/kg) and AC (5 mg/kg and 10 mg/kg) were orally administered to rats with acetic acid-induced gastric ulcers. They were found to reduce the size of the ulcers at days 1, 3 and 7 after ulcer induction in a dose-dependent manner, with a concomitant attenuation of iNOS activity and protein expression at the ulcer tissues. The levels of nitrite and nitrate (NOX -), the stable end-products of nitric oxide (NO), in the gastric ulcer tissues were also decreased. N-[3-(aminomethyl)benzyl]acetamidine (1400W), a highly selective inhibitor of iNOS, was found to produce similar but more potent inhibition on iNOS activity at a dose of 0.1 mg/kg. These findings indicate that CE and AC have an anti-inflammatory property that is brought about by inhibition of NO synthesis and thus facilitates ulcer healing.
Abbreviations
CE:Centella asiatica water extract
AC:asiaticoside
iNOS:inducible nitric oxide synthase
NO:nitric oxide
NOx-:nitrite and nitrate
1400W:N-[3-(aminomethyl)benzyl]acetamidine
HPLC:high performance liquid chromatography
Key words
Centella asiatica - Apiaceae - Asiaticoside - inducible nitric oxide synthase - gastric ulcer
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Dr. M. W. L. Koo
Department of Pharmacology
Faculty of Medicine
The University of Hong Kong
21 Sassoon Road
Pokfulam
Hong Kong
P. R. China
Telefon: +852-2819-9256
Fax: +852-2817-0859
eMail: wlkoo@hkusua.hku.hk