Planta Med 2005; 71(3): 219-224
DOI: 10.1055/s-2005-837820
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Antitumor Effects of Zerumbone from Zingiber zerumbet in P-388D1 Cells in Vitro and in Vivo

Guan-Cheng Huang1 , Ting-Yi Chien2 , Lih-Geeng Chen3 , Ching-Chiung Wang2
  • 1Department of Internal Medicine, Chi-Mei Medical Center, Tainan, Taiwan, R.O.C.
  • 2School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan, R.O.C.
  • 3Graduate Institute of Biopharmaceutics, College of Life Science, National Chiayi University, Chiayi, Taiwan, R.O.C.
Further Information

Publication History

Received: June 9, 2004

Accepted: October 24, 2004

Publication Date:
15 March 2005 (online)

Abstract

The fresh rhizome of Zingiber zerumbet (L.) Roscoe ex Smith (Zingiberaceae) is widely used as a folk medicine in Taiwan. In this study, the fresh rhizome was extracted with 95 % EtOH and partitioned with diethyl ether. The antitumor effects of the diethyl ether extract were measured in cultured P-388D1 cells and in an animal model of P-388D1-bearing CDF1 mice. The results indicated that the extract could induce DNA fragmentation in P-388D1 cells in vitro, and significantly prolong the life of P-388D1-bearing CDF1 mice (ILS% = 127.8) at a dosage of 5 mg/kg body weight. After column chromatography combined with an MTT cytotoxicity bioassay, zerumbone, a cyclic sesquiterpene was isolated from the diethyl ether extract. Zerumbone inhibited the growth of P-388D1 cells, induced DNA fragmentation in culture, and significantly prolonged the life of P-388D1-bearing CDF1 mice (ILS% = 120.5) at a dosage of 2 mg/kg. Furthermore, zerumbone inhibited the growth of a human leukemia cell line, HL-60 cells, in a time- and concentration-dependent manner, with IC50 values of 22.29, 9.12, and 2.27 μg/mL for 6, 12, and 18 h, respectively. The cell cycle of HL-60 cells was observed after treatment with zerumbone, which induced G2/M cell cycle arrest in HL-60 cells in a time- and concentration-dependent manner, and decreased the cyclin B1/cdk 1 protein level. These results suggest that zerumbone is an active principal of Z. zerumbet and is potentially a lead compound for the development of anticancer drugs.

References

Prof. Ching-Chiung Wang

School of Pharmacy, College of Pharmacy, Taipei Medical University

250 Wu-Hsing Street

Taipei 110

Taiwan, R.O.C.

Phone: 886-2-27361661 ext. 6161

Fax: 886-2-27388351

Email: crystal@tmu.edu.tw