Immunomodulatory Activity and Influence on Mitotic Divisions of N-Benzoylphenylisoserinates of Lactarius Sesquiterpenoid Alcohols in vitro

Abstract

Six N-benzoylphenylisoserinates of Lactarius sesquiterpenoid alcohols, which previously showed antiviral activities, were tested for their biological properties. Their influence on the mitotic division of the cells and on selected immunological parameters, e. g., T and B lymphocyte proliferation and synthesis of the cytokines: interleukin 2 (IL-2), tumour necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) was assessed in vitro. All of the tested compounds significantly decreased the number of cell divisions. It appears that their influence on cellular divisions may be associated with anti-HSV activity. Moreover, one compound - isolactarorufin 8-epi-[N-benzoyl-(2′R,3′S)-3′-phenylisoserinate] significantly inhibited T lymphocyte proliferation and the synthesis of all tested cytokines.

Abbreviations

DMSO:dimethyl sulphoxide

FCS:foetal calf serum

HEPES:4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid

MEM:minimum essential medium

PBMC:peripheral blood mononuclear cells

PBS:phosphate buffered saline

PHA:phytohaemagglutinin

PMA:phorbol myristate acetate

SAC:Staphylococcus aureus Cowan (strain)

Key words

Sesquiterpenes - taxol - Lactarius - mitotic divisions - lymphocyte proliferation - cytokines

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