Abstract
The production of five antifungal saponins (SC-2 - SC-6) recently extracted from Solanum chrysotrichum leaves was performed using hairy root cultures grown in 250-mL flasks and in 2-L
modified draught-tube internal-loop airlift reactors (2-L MR). In both scales, first
order growth kinetics were observed, reaching specific growth rates of 0.08 and 0.12
d-1, respectively. Root density at the end of the culture period was the same for the
flasks and for the 2-L MR. In flasks the production of the most active saponin SC-2
was growth-associated, with a maximum yield of 0.04 % dry weight, while in the 2-L
MR an SC-2 yield of 0.7 % dry weight was reached, representing a value six times greater
than that observed for plant leaves. SC-3 was obtained from root biomasses grown in
flasks, while SC-4 was recovered from biomasses at both levels under investigation.
SC-5 and SC-6 were only detected in the culture medium of roots grown in 2-L MR. Solanum chrysotrichum hairy root cultures and their scale-up in reactors are feasible strategies for the
production of these antifungal compounds for human use.
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Ma. Luisa Villarreal
Centro de Investigación en Biotecnología
Universidad Autónoma del Estado de Morelos
Cuernavaca 62210
Morelos
México
Phone: /Fax: +52-777-329-7057
Email: luisav@cib.uaem.mx
