Planta Med 2006; 72(7): 650-652
DOI: 10.1055/s-2006-931535
© Georg Thieme Verlag KG Stuttgart · New York

Molecular Identification of Sinopodophyllum hexandrum and Dysosma Species using cpDNA Sequences and PCR-RFLP Markers

Wei Gong1 , Cheng-Xin Fu1 , Yu-Ping Luo1 , Ying-Xiong Qiu1
  • 1Laboratory of Systematic and Evolutionary Botany, Department of Biology, College of Life Sciences, Zhejiang University, Hangzhou 310029, P. R. China
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Received: October 26, 2005

Accepted: December 7, 2005

28. April 2006 (online)


Rhizomes of Sinopodophyllum hexandrum and Dysosma species, which have long been used in the traditional Chinese herbal medicine, have similar morphology and chemical composition. However, the podophyllotoxin content is higher in the rhizomes of S. hexandrum than in those of Dysosma species. The PCR-amplified fragments of trnT-trnL showed length variation between S. hexandrum and Dysosma species, and sequence comparison indicated that the length variation resulted from differential indels. There were species-specific PCR-RFLP markers of the chloroplast trnD-trnT region. Our results suggest that both chloroplast intergenic regions can be used for the identification of S. hexandrum and Dysosma Rhizoma medicines on the market.


Ying-Xiong Qiu

Laboratory of Systematic and Evolutionary Botany

Department of Biology

College of Life Sciences

Zhejiang University

Hangzhou 310029

People’s Republic of China

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